Pig C-Peptide ELISA Kit

Code CSB-E15977p
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation C-Peptide
Target Name C-Peptide
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 ng/mL-80 ng/mL
Sensitivity 0.312 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %88-98
1:2Average %96
Range %91-102
1:4Average %103
Range %97-107
1:8Average %87
Range %81-93
Recovery
The recovery of pig C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9793-104
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
802.663 2.562 2.613 2.487
402.093 2.214 2.154 2.028
201.510 1.531 1.521 1.395
101.043 1.074 1.059 0.933
50.634 0.623 0.629 0.503
2.50.381 0.370 0.376 0.250
1.250.248 0.244 0.246 0.120
00.127 0.125 0.126  
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