Rabbit C-Peptide ELISA kit

Code CSB-EQ027310RB
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01311
Lead Time 7-14 working days
Abbreviation C-Peptide
Protein Biological Process 1 Metabolism
Target Name C-Peptide
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 0.5 ng/mL-15 ng/mL
Sensitivity 0.3 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-103
1:2Average %95
Range %91-99
1:4Average %98
Range %93-104
1:8Average %87
Range %81-92
Recovery
The recovery of rabbit C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-97
EDTA plasma (n=4)9792-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
151.954 1.905 1.930 1.895
81.432 1.358 1.395 1.360
40.782 0.791 0.787 0.752
20.414 0.428 0.421 0.386
0.50.168 0.164 0.166 0.131
00.034 0.035 0.035
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Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: ocu:100009181

STRING: 9986.ENSOCUP00000020463

UniGene: Ocu.1810

Pathway HIF-1 signaling pathway
MAPK signaling pathway
mTOR signaling pathway
PI3K-Akt signaling pathway
Regulation of actin cytoskeleton
Autophagy
Ras signaling pathway
AMPK signaling
cGMP-PKG signaling pathway
FoxO signaling pathway
Insulin signaling pathway
Phospholipase D signaling pathway
Rap1 signaling pathway

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