Pig Colipase(CLPS) ELISA kit

Code CSB-EL005578PI
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
colipase, pancreatic
Alternative Names
CLPS ELISA Kit; Colipase ELISA Kit; Procolipase II ELISA Kit
Abbreviation
CLPS
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 mU/mL-1000 mU/mL
Sensitivity
3.9 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig CLPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 82-92
1:2 Average % 99
Range % 94-103
1:4 Average % 103
Range % 98-107
1:8 Average % 95
Range % 90-101
Recovery
The recovery of pig CLPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-105
EDTA plasma (n=4) 104 88-111
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
1000 2.297 2.312 2.305 2.196
500 1.786 1.688 1.737 1.628
250 1.133 1.202 1.168 1.059
125 0.654 0.665 0.660 0.551
62.5 0.389 0.374 0.382 0.273
31.2 0.242 0.254 0.248 0.139
15.6 0.177 0.187 0.182 0.073
0 0.105 0.113 0.109  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig CLPS ELISA Kit was designed for the quantitative measurement of Pig CLPS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 mU/mL-1000 mU/mL and the sensitivity is 3.9 mU/mL.

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Target Background

Function
(From Uniprot)
Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase.; Enterostatin has a biological activity as a satiety signal.
Gene References into Functions
  1. isotropic orientation of colipase which could explain its incapacity to reverse the inhibitory effects of these lipids on the lipase activity PMID: 18052211
  2. Analysis of structures of NMR colipase models and simulations of their interactions with various lipid aggregates, lipid droplet, and bile salt micelle, were carried out to determine and to map the lipid binding sites on colipase. PMID: 18506778
Subcellular Location
Secreted.
Protein Families
Colipase family
Tissue Specificity
Expressed by the pancreas.
Database Links
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