Pig Corticoliberin(CRH) ELISA kit

Code CSB-EL005963PI
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
corticotropin releasing hormone
Alternative Names
CRHCorticoliberin ELISA kit; Corticotropin-releasing factor ELISA kit; CRF ELISA kit; Corticotropin-releasing hormone ELISA kit
Abbreviation
CRH
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
100 pg/mL-1600 pg/mL
Sensitivity
100 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig CRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 85-95  
1:2 Average % 95  
Range % 92-101  
1:4 Average % 82  
Range % 80-83  
1:8 Average % 88  
Range % 85-92  
Recovery
The recovery of pig CRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 87-97  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1600 0.196 0.184 0.190    
800 0.322 0.343 0.333    
400 0.516 0.510 0.513    
200 1.037 1.100 1.069    
100 2.158 2.323 2.241    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig CRH ELISA Kit was designed for the quantitative measurement of Pig CRH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 100 pg/mL-1600 pg/mL and the sensitivity is 100 pg/mL.

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Target Background

Function
(From Uniprot)
Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile.
Gene References into Functions
  1. Evidence is provided for porcine corticotropin releasing hormone (CRH) as a quantitative trait locus (QTL) affecting growth and body composition. (corticotropin releasing hormone; CRH; CRF) PMID: 16483545
  2. CRH inhibits local progesterone production from luteal cells in swine corpus lutem. PMID: 20431270
Subcellular Location
Secreted.
Protein Families
Sauvagine/corticotropin-releasing factor/urotensin I family
Tissue Specificity
Produced by the hypothalamus.
Database Links
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