Pig Epithelial growth factor,EGF ELISA Kit

Instructions
Code CSB-E06788p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Epithelial growth factor,EGF
Abbreviation EGF
Uniprot No. F1S137
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 250 pg/mL-10000 pg/mL
Sensitivity 125 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 100  
Range % 97-105  
1:2 Average % 93  
Range % 90-96  
1:4 Average % 102  
Range % 98-106  
1:8 Average % 87  
Range % 83-90  
Recovery
The recovery of pig EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 83-89  
EDTA plasma (n=4) 101 99-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
10000 2.647 2.687 2.667    
5000 1.839 1.899 1.869    
2500 1.057 1.067 1.062    
1250 0.599 0.584 0.592    
500 0.241 0.231 0.236    
250 0.112 0.114 0.113    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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