Pig Epithelial growth factor,EGF ELISA Kit

Code CSB-E06788p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Epithelial growth factor,EGF
Abbreviation
EGF
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
250 pg/mL-10000 pg/mL
Sensitivity
125 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 100  
Range % 97-105  
1:2 Average % 93  
Range % 90-96  
1:4 Average % 102  
Range % 98-106  
1:8 Average % 87  
Range % 83-90  
Recovery
The recovery of pig EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 83-89  
EDTA plasma (n=4) 101 99-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
10000 2.647 2.687 2.667    
5000 1.839 1.899 1.869    
2500 1.057 1.067 1.062    
1250 0.599 0.584 0.592    
500 0.241 0.231 0.236    
250 0.112 0.114 0.113    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig EGF ELISA Kit was designed for the quantitative measurement of Pig EGF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 250 pg/mL-10000 pg/mL and the sensitivity is 125 pg/mL.

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