Pig Interleukin 1 receptor antagonist, IL-1ra ELISA kit

Code CSB-E04946p
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
interleukin 1 receptor antagonist
Alternative Names
IL1RN ELISA kit; IRAP1Interleukin-1 receptor antagonist protein ELISA kit; IL-1RN ELISA kit; IL-1ra ELISA kit; IRAP ELISA kit; IL1 inhibitor ELISA kit
Abbreviation
IL1RN
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 106
Range % 93-110
1:2 Average % 101
Range % 91-105
1:4 Average % 97
Range % 87-101
1:8 Average % 91
Range % 84-95
Recovery
The recovery of pig IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 85-96
EDTA plasma (n=4) 108 98-112
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 2.243 2.187 2.215 2.032
2000 1.441 1.366 1.404 1.221
1000 0.986 0.954 0.970 0.787
500 0.658 0.647 0.653 0.470
250 0.422 0.402 0.412 0.229
125 0.352 0.343 0.348 0.165
62.5 0.277 0.263 0.270 0.087
0 0.186 0.180 0.183  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig IL1RN ELISA Kit was designed for the quantitative measurement of Pig IL1RN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Target Background

Function
(From Uniprot)
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
Gene References into Functions
  1. This study identified IL-1Ra as an early immunomodulatory mediator that could be involved in the immunopathogenesis of porcine reproductive and respiratory syndrome virus infections. PMID: 27902420
Subcellular Location
Secreted.
Protein Families
IL-1 family
Database Links

UniGene: Ssc.16250

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