Pig Interleukin 22(IL-22) ELISA Kit

Code CSB-E15847p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Pig IL-22 ELISA Kit was designed for the quantitative measurement of Pig IL-22 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

Target Name Interleukin 22(IL-22)
Abbreviation IL-22
Uniprot No. F1SH68
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 99
Range % 94-104
1:2 Average % 96
Range % 89-100
1:4 Average % 95
Range % 89-98
1:8 Average % 103
Range % 95-107
Recovery
The recovery of pig IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 91-103
EDTA plasma (n=4) 101 95-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.162 2.085 2.124 1.948
2500 1.875 1.811 1.843 1.667
1250 1.469 1.383 1.426 1.250
625 0.787 0.764 0.776 0.600
312 0.484 0.472 0.478 0.302
156 0.315 0.304 0.310 0.134
78 0.231 0.228 0.230 0.054
0 0.178 0.174 0.176  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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