| Description |
This Pig IL-22 ELISA Kit was designed for the quantitative measurement of Pig IL-22 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL. |
| Target Name |
Interleukin 22(IL-22) |
| Abbreviation |
IL-22 |
| Uniprot No. |
F1SH68 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
78 pg/mL-5000 pg/mL |
| Sensitivity |
19.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | | Sample | Serum(n=4) | | | | 1:1 | Average % | 99 | | | | Range % | 94-104 | | | | 1:2 | Average % | 96 | | | | Range % | 89-100 | | | | 1:4 | Average % | 95 | | | | Range % | 89-98 | | | | 1:8 | Average % | 103 | | | | Range % | 95-107 | | |
|
| Recovery |
| The recovery of pig IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | | | | Sample Type | Average % Recovery | Range | | | | Serum (n=5) | 99 | 91-103 | | | | EDTA plasma (n=4) | 101 | 95-107 | | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | | |  | pg/ml | OD1 | OD2 | Average | Corrected | | | | 5000 | 2.162 | 2.085 | 2.124 | 1.948 | | | | 2500 | 1.875 | 1.811 | 1.843 | 1.667 | | | | 1250 | 1.469 | 1.383 | 1.426 | 1.250 | | | | 625 | 0.787 | 0.764 | 0.776 | 0.600 | | | | 312 | 0.484 | 0.472 | 0.478 | 0.302 | | | | 156 | 0.315 | 0.304 | 0.310 | 0.134 | | | | 78 | 0.231 | 0.228 | 0.230 | 0.054 | | | | 0 | 0.178 | 0.174 | 0.176 | | | |
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
