Pig Keratinocyte Growth Factor,KGF ELISA Kit

Pig Keratinocyte Growth Factor,KGF ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

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Product Details

Citations

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Customer Reviews and Q&A

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Pig Keratinocyte Growth Factor,KGF ELISA Kit

Citations (1) Protocol MSDS

Code	CSB-E06800p
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (1)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

fibroblast growth factor 7 (keratinocyte growth factor)

Alternative Names

FGF7Fibroblast growth factor 7 ELISA kit; FGF-7 ELISA kit; Heparin-binding growth factor 7 ELISA kit; HBGF-7 ELISA kit; Keratinocyte growth factor ELISA kit; KGF ELISA kit

Abbreviation

FGF7

Uniprot No.

Q9N198

Species

Sus scrofa (Pig)

Sample Types

serum, plasma

Detection Range

3.12 pg/mL-200pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	88
	Range %	84-92
1:4	Average %	90
	Range %	85-98
1:8	Average %	106
	Range %	101-114

Recovery

The recovery of pig KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	84	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	1.815	1.731	1.773	1.632
100	1.128	1.173	1.151	1.010
50	0.789	0.827	0.808	0.667
25	0.503	0.526	0.515	0.374
12.5	0.312	0.309	0.311	0.170
6.25	0.251	0.271	0.261	0.120
3.12	0.188	0.178	0.183	0.042
0	0.144	0.138	0.141

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Pig FGF7 ELISA Kit was designed for the quantitative measurement of Pig FGF7 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200pg/mL and the sensitivity is 0.78 pg/mL.

Characterization of bladder acellular matrix hydrogel with inherent bioactive factors Dan Jiang.et al,Materials Science and Engineering: C,2017

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Function
(From Uniprot)

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.

Subcellular Location

Secreted.

Protein Families

Heparin-binding growth factors family

Database Links

STRING: 9823.ENSSSCP00000025433

UniGene: Ssc.15923

Promotions

Purchase Promotion 96T ELISA Kit, Receive $30 Amazon Gift Card

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Related pathways

MAPK signaling pathway PI3K-Akt signaling pathway Regulation of actin cytoskeleton Ras signaling pathway Rap1 signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.