Pig Platelet-Derived Growth Factor AB (PDGF-AB) ELISA kit

Code CSB-EQ027978PI
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Platelet-Derived Growth Factor AB (PDGF-AB)
Abbreviation
PDGF-AB
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 85-103
1:2 Average % 90
Range % 85-99
1:4 Average % 93
Range % 84-97
1:8 Average % 95
Range % 83-100
Recovery
The recovery of pig PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 83-101
EDTA plasma (n=4) 93 87-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.410 2.453 2.432 2.250
10 1.573 1.565 1.569 1.387
5 0.988 0.997 0.993 0.811
2.5 0.609 0.621 0.615 0.433
1.25 0.429 0.438 0.434 0.252
0.625 0.347 0.335 0.341 0.159
0.312 0.245 0.242 0.244 0.062
0 0.180 0.183 0.182  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig PDGF-AB ELISA Kit was designed for the quantitative measurement of Pig PDGF-AB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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