Pig Thyroid Stimulating Hormone,TSH ELISA Kit

Code CSB-E13719p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Thyroid Stimulating Hormone,TSH
Alternative Names
TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
Abbreviation
TSH
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma
Detection Range
0.6 μIU/mL-24 μIU/mL
Sensitivity
0.3 μIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %90-101
1:2Average %93
Range %88-97
1:4Average %102
Range %98-106
1:8Average %88
Range %82-94
Recovery
The recovery of pig TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-99
EDTA plasma (n=4)10298-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/mlOD1OD2Average
242.354 2.219 2.287
121.252 1.215 1.234
60.635 0.648 0.642
30.327 0.310 0.319
1.20.155 0.152 0.154
0.60.079 0.078 0.079
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig TSH ELISA Kit was designed for the quantitative measurement of Pig TSH protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.6 μIU/mL-24 μIU/mL and the sensitivity is 0.3 μIU/mL.

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Target Background

Function
(From Uniprot)
Indispensable for the control of thyroid structure and metabolism.
Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit beta family
Database Links
CUSABIO guaranteed quality
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