Pig Thyroid Stimulating Hormone,TSH ELISA Kit

Instructions
Code CSB-E13719p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Thyroid Stimulating Hormone,TSH
Alternative Names TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
Abbreviation TSH
Uniprot No. P01224
Species Sus scrofa (Pig)
Sample Types serum, plasma
Detection Range 0.6 μIU/mL-24 μIU/mL
Sensitivity 0.3 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %90-101
1:2Average %93
Range %88-97
1:4Average %102
Range %98-106
1:8Average %88
Range %82-94
Recovery
The recovery of pig TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-99
EDTA plasma (n=4)10298-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/mlOD1OD2Average
242.354 2.219 2.287
121.252 1.215 1.234
60.635 0.648 0.642
30.327 0.310 0.319
1.20.155 0.152 0.154
0.60.079 0.078 0.079
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Indispensable for the control of thyroid structure and metabolism.
Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: ssc:397658

STRING: 9823.ENSSSCP00000007199

UniGene: Ssc.14053

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