Pig cholecystokinin,CCK ELISA Kit

Instructions
Code CSB-E10131p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name cholecystokinin
Alternative Names CCK ELISA Kit; Cholecystokinin ELISA Kit; CCK) [Cleaved into: Cholecystokinin-58 ELISA Kit; CCK58); Cholecystokinin-58 desnonopeptide ELISA Kit; (1-49)-CCK58); Cholecystokinin-39 ELISA Kit; CCK39); Cholecystokinin-33 ELISA Kit; CCK33); Cholecystokinin-25 ELISA Kit; CCK25); Cholecystokinin-18 ELISA Kit; CCK18); Cholecystokinin-12 ELISA Kit; CCK12); Cholecystokinin-8 ELISA Kit; CCK8); Cholecystokinin-7 ELISA Kit; CCK7); Cholecystokinin-5 ELISA Kit; CCK5)] ELISA Kit
Abbreviation CCK
Uniprot No. P01356
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 93-100  
1:2 Average % 104  
Range % 101-107  
1:4 Average % 91  
Range % 85-97  
1:8 Average % 90  
Range % 87-93  
Recovery
The recovery of pig CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 85 81-89  
EDTA plasma (n=4) 92 87-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.179 2.198 2.189 2.031  
50 1.698 1.677 1.688 1.530  
25 1.136 1.166 1.151 0.993  
12.5 0.697 0.658 0.678 0.520  
6.25 0.486 0.497 0.492 0.334  
3.12 0.383 0.398 0.391 0.233  
1.56 0.301 0.321 0.311 0.153  
0 0.157 0.159 0.158    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
Gene References into Functions
  1. We conclude that only a small fraction of neuronal CCK is nonsulfated. The intracellular distribution of nonsulfated CCK in neurons suggests that they contribute only modestly to the CCK transmitter activity. PMID: 27535680
  2. Cardiac expression of pro-cholecystokinin is cell-specific, which differentiates the expression from that of intestinal endocrine cells and cerebral neurons. PMID: 25627687
  3. This review explores the role of luminal cholecystokinin on the regulation of exocrine pancreatic secretion in author's studies of neonatal pigs. PMID: 15075451
Subcellular Location Secreted
Protein Families Gastrin/cholecystokinin family
Tissue Specificity Synthesized in both cerebral cortex and duodenal mucosa.
Database Links

KEGG: ssc:397468

STRING: 9823.ENSSSCP00000012023

UniGene: Ssc.717

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1