Pig collagen type I (COLI) ELISA kit

Instructions
Code CSB-EQ027853PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name collagen type I (COLI)
Alternative Names collagen type 1 (COL1) ,collagen type I (COLI)
Abbreviation COLI
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig COLⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 88    
Range % 86-91    
1:2 Average % 89    
Range % 81-95    
1:4 Average % 98    
Range % 93-104    
1:8 Average % 102    
Range % 98-106    
Recovery
The recovery of pig COLⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 104 94-109    
EDTA plasma (n=4) 92 88-98    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
5000 2.621 2.731 2.676 2.501    
2500 2.155 2.177 2.166 1.991    
1250 1.503 1.455 1.479 1.304    
625 0.983 0.930 0.957 0.782    
312 0.672 0.696 0.684 0.509    
156 0.451 0.444 0.448 0.273    
78 0.315 0.329 0.322 0.147    
0 0.174 0.176 0.175      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

I would like to confirm that the COL1 ELISA Kit, is specific for pig collagen type 1

A:
Thanks for your inquiry!The kit csb-eq027853pi specifically detects pig collagen type I.

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  Email: [email protected]
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