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Pig collagen type I (COLI) ELISA kit

Pig collagen type I (COLI) ELISA kit

Citations (1) Protocol MSDS

Code	CSB-EQ027853PI
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details
Citations (1)
Customer Reviews and Q&A

Product Details

Target Name

collagen type I (COLI)

Alternative Names

collagen type 1 (COL1) ,collagen type I (COLI)

Abbreviation

COLI

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig COLⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	86-91
1:2	Average %	89
	Range %	81-95
1:4	Average %	98
	Range %	93-104
1:8	Average %	102
	Range %	98-106

Recovery

The recovery of pig COLⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	94-109
EDTA plasma (n=4)	92	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.621	2.731	2.676	2.501
2500	2.155	2.177	2.166	1.991
1250	1.503	1.455	1.479	1.304
625	0.983	0.930	0.957	0.782
312	0.672	0.696	0.684	0.509
156	0.451	0.444	0.448	0.273
78	0.315	0.329	0.322	0.147
0	0.174	0.176	0.175

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Pig COLI ELISA Kit was designed for the quantitative measurement of Pig COLI protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

Citations

Preclinical efficacy study of a porous biopolymeric scaffold based on(gelatin-hyaluronic acid-chondroitin sulfate) in a porcine burn injury model: Role of critical molecular markers (VEGFA, N-Cadherin, COX-2), gamma sterilization efficacy and a comparison Amit Khurana,Biomed Mater,2021

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I would like to confirm that the COL1 ELISA Kit, is specific for pig collagen type 1

Thanks for your inquiry!The kit csb-eq027853pi specifically detects pig collagen type I.

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