Pig hepatocyte growth factor,HGF ELISA Kit

Code CSB-E06795p
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. F1SB93
Lead Time 3-5 working days
Abbreviation HGF
Target Name hepatocyte growth factor,HGF
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 84-92  
1:2 Average % 104  
Range % 94-110  
1:4 Average % 95  
Range % 89-99  
1:8 Average % 94  
Range % 86-97  
Recovery
The recovery of pig HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 90-95  
EDTA plasma (n=4) 97 92-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.894 2.794 2.844 2.683  
2000 2.344 2.244 2.294 2.133  
1000 1.524 1.474 1.499 1.338  
500 0.954 0.924 0.939 0.778  
250 0.556 0.546 0.551 0.390  
125 0.371 0.361 0.366 0.205  
62.5 0.240 0.230 0.235 0.074  
0 0.162 0.160 0.161    
References
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