Pig interleukin 10,IL-10 ELISA Kit

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Pig interleukin 10,IL-10 ELISA Kit

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Protocol

Code	CSB-E06779p-IS
Size	96T,5×96T,10×96T
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Product Details
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Customer Reviews and Q&A
Target Background

Target Name

interleukin 10

Associated names

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit

Abbreviation

IL10

Uniprot No.

Q29055

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8pg/mL-500pg/mL

Sensitivity

3.9pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	95
	Range %	89-101
1:4	Average %	97
	Range %	90-104
1:8	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
500	2.034	2.134	2.084	1.910
250	1.444	1.467	1.456	1.281
125	0.928	0.917	0.922	0.748
62.5	0.580	0.572	0.576	0.402
31.2	0.399	0.381	0.390	0.216
15.6	0.319	0.303	0.311	0.137
7.8	0.226	0.234	0.230	0.056
0	0.174	0.175	0.174

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

Description

This ELISA kit provides quantitative measurement of interleukin-10 (IL-10) in porcine samples, supporting immunological research where IL-10 serves as a critical anti-inflammatory and immunosuppressive cytokine. Because porcine IL-10 levels can shift dramatically between baseline immunosuppressive signaling and acute inflammatory responses such as PRRSV infection or LPS challenge, precise quantification across this dynamic range is essential.

Detection Range: The 7.8–500 pg/mL range captures both constitutive IL-10 production in healthy porcine serum and the elevated concentrations observed during active immune suppression or post-infection cytokine storms, minimizing the need for serial dilutions in most in vivo challenge studies.

Sensitivity: At 3.9 pg/mL, the kit detects the low basal IL-10 levels characteristic of unstimulated porcine plasma and early-phase immune responses, where IL-10 upregulation may be subtle yet biologically decisive in polarizing macrophage phenotype.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables researchers to correlate systemic circulating IL-10 with localized immunosuppressive microenvironments in gut, lung, or lymph node tissue — critical for mucosal immunology and transplant tolerance studies in the pig model.

Assay Efficiency: The 50–100 µL sample volume and 1–5 hour protocol accommodate limited-volume piglet bleeds and multi-cytokine panel designs common in vaccination time-course experiments.

This kit is particularly suited for porcine reproductive and respiratory syndrome research, swine vaccine development, and gut-associated immune tolerance studies.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.

Gene References into Functions

The PRRSV vaccination induced higher levels of IL-10 expression in the first week and this may be why the CSFV vaccination is immunosuppressed. PMID: 27938685
These data indicate that the type 2 porcine reproductive and respiratory syndrome virus N protein plays an important role in IL-10 induction and the N-N non-covalent domain is associated with this activity. PMID: 25614594
The effects of semen, spermatozoa in extender, or extender alone on the expression of TGF-beta1, IL-10, and IL-6 in ovarian follicles are reported. PMID: 24020676
Prostaglandin E2 potentiates mesenchymal stem cell-induced IL-10+IFN-gamma+CD4+ regulatory T cells to control transplant arteriosclerosis. PMID: 23359497
Porcine reproductive and respiratory syndrome virus strain CH-1a could significantly up-regulate IL-10 production through p38 MAPK activation. PMID: 22909062
Boar seminal plasma contained TGF- beta1 and IL-10 but with high individual variation. PMID: 22482797
Changes in interleukin-10 mRNA expression are predictive for 9-day survival of pigs in an emergency preservation and resuscitation model. PMID: 20163907
These results suggest that TNF-alpha and IL-10, but not IL-6, are involved in the late reparatory phases of the experimental disk lesion. PMID: 19605905
The present study shows that elevated levels of serum CRP and IL-10 were associated with porcine circovirus type 2-infected piglets that subsequently developed severe PMWS.(postweaning multisystemic wasting syndrome) PMID: 16817761
Subclinically porcine circovirus type 2 (PCV2)-infected pigs developed a transient PCV2-specific IL-10 response during the viremic phase of infection which coincided with the inversion of the IgM/IgG ratio. PMID: 17592737
Twenty one polymorphisms in the IL10 gene have been found, including single nucleotide polymorphisms and insertion deletion polymorphisms. The chromosomal location has been mapped by FISH and radiation hybrid mapping. PMID: 18607788
findings show that Brachyspira hyodysenteriae inoculation induced production of systemic levels of IL-1beta during the dysentery period and increased levels of IL-10 coincided with recovery from dysentery PMID: 18700003

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Subcellular Location

Secreted.

Protein Families

IL-10 family

Database Links

KEGG: ssc:397106

STRING: 9823.ENSSSCP00000016594

UniGene: Ssc.148

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Email: [email protected]
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Related pathways

Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway

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