Pig interleukin 10,IL-10 ELISA Kit

Product Details

Customer Reviews and Q&A

Target Background

Home
Kit
ELISA Kits
Pig interleukin 10,IL-10 ELISA Kit

^Promotion

Protocol

Code	CSB-E06779p-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Target Name

interleukin 10

Alternative Names

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit

Abbreviation

IL10

Uniprot No.

Q29055

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8pg/mL-500pg/mL

Sensitivity

3.9pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	95
	Range %	89-101
1:4	Average %	97
	Range %	90-104
1:8	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
500	2.034	2.134	2.084	1.910
250	1.444	1.467	1.456	1.281
125	0.928	0.917	0.922	0.748
62.5	0.580	0.572	0.576	0.402
31.2	0.399	0.381	0.390	0.216
15.6	0.319	0.303	0.311	0.137
7.8	0.226	0.234	0.230	0.056
0	0.174	0.175	0.174

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.

Gene References into Functions

The PRRSV vaccination induced higher levels of IL-10 expression in the first week and this may be why the CSFV vaccination is immunosuppressed. PMID: 27938685
These data indicate that the type 2 porcine reproductive and respiratory syndrome virus N protein plays an important role in IL-10 induction and the N-N non-covalent domain is associated with this activity. PMID: 25614594
The effects of semen, spermatozoa in extender, or extender alone on the expression of TGF-beta1, IL-10, and IL-6 in ovarian follicles are reported. PMID: 24020676
Prostaglandin E2 potentiates mesenchymal stem cell-induced IL-10+IFN-gamma+CD4+ regulatory T cells to control transplant arteriosclerosis. PMID: 23359497
Porcine reproductive and respiratory syndrome virus strain CH-1a could significantly up-regulate IL-10 production through p38 MAPK activation. PMID: 22909062
Boar seminal plasma contained TGF- beta1 and IL-10 but with high individual variation. PMID: 22482797
Changes in interleukin-10 mRNA expression are predictive for 9-day survival of pigs in an emergency preservation and resuscitation model. PMID: 20163907
These results suggest that TNF-alpha and IL-10, but not IL-6, are involved in the late reparatory phases of the experimental disk lesion. PMID: 19605905
The present study shows that elevated levels of serum CRP and IL-10 were associated with porcine circovirus type 2-infected piglets that subsequently developed severe PMWS.(postweaning multisystemic wasting syndrome) PMID: 16817761
Subclinically porcine circovirus type 2 (PCV2)-infected pigs developed a transient PCV2-specific IL-10 response during the viremic phase of infection which coincided with the inversion of the IgM/IgG ratio. PMID: 17592737
Twenty one polymorphisms in the IL10 gene have been found, including single nucleotide polymorphisms and insertion deletion polymorphisms. The chromosomal location has been mapped by FISH and radiation hybrid mapping. PMID: 18607788
findings show that Brachyspira hyodysenteriae inoculation induced production of systemic levels of IL-1beta during the dysentery period and increased levels of IL-10 coincided with recovery from dysentery PMID: 18700003

Hide All

Subcellular Location

Secreted.

Protein Families

IL-10 family

Database Links

KEGG: ssc:397106

STRING: 9823.ENSSSCP00000016594

UniGene: Ssc.148

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

STAT3: a Key Transcriptional Regulator in Cancer Two Types of Macrophages: M1 and M2 Macrophages Surface Markers That Help You Identify Lymphocytes Surface Markers of Mesenchymal Stem Cells Interleukin 4 receptor (IL-4R/IL-4Ra): a Multifunctional Target of Cytokines, Suggesting Diverse Potential in Asthma, Rhinitis, Viral, and Tumor Research! CEACAM8: a Member of CEA Family, Emerging as a Vital Player in Gynaecological or Gastrointestinal Cancer Research! The Metabolic Phenotype of Tumor-Associated Macrophages in the Tumor Microenvironment CD147, a New Target of SARS-CoV-2 Invasion LPS/TLR4 Signal Transduction Pathway What You Have to Know about Cytokine Storm and Virus Infection VSIG4: a Prominent B7 Family Costimulatory Molecule or Complement Receptor in Cancers and Inflammatory Diseases! How do Th1 and Th2 Cells Differentiate? Identify Treg Cells. You Can Do This. PD-1-- An Important Immune Checkpoint Surface Markers of Natural Killer Cells Another powerful addition to domestic IL-17A inhibitors! 3S Guojian's Anmoqitamab approved, with once-every-8-week dosing—a game-changer for psoriasis patients. CD69: a Classical Early Marker of Lymphocyte Activation, a Pivotal Regulator of Hematologic Diseases and Autoimmune Disorders! Itaconate is a negative regulator of inflammation The Overview of Interferon The Overview of Interleukin Chemokine Receptor CCR6: the Specific Receptor for CCL20, a Potential Target for Drug Research in Inflammatory Diseases and Gastrointestinal Cancer! The "Plaque Cleaner" in Blood Vessels Has Arrived! CD25+ Regulatory T Cells Transform into CAR-Tregs with Amazing Results CNR1 (CB1): A Crucial Receptor of Endocannabinoid System Offers Potential Treatments for Tumors and Glycolipid Metabolism Disorders! Targeting LRRC15: From tumor microenvironment regulation to fibrotic/infectious disease therapy How the Tumor Microenvironment Fuels Cancer's Deadly Evolution ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Related pathways

Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address