Pig interleukin 10,IL-10 ELISA Kit

Instructions
Code CSB-E06779p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 10
Alternative Names IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
Abbreviation IL10
Uniprot No. Q29055
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %81-103
1:2Average %95
Range %90-105
1:4Average %95
Range %90-108
1:8Average %96
Range %82-112
Recovery
The recovery of pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9585-109
EDTA plasma (n=4)9792-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.178 2.152 2.165 2.015
2001.584 1.560 1.572 1.422
1000.981 0.950 0.966 0.816
500.568 0.564 0.566 0.416
250.372 0.361 0.367 0.217
12.50.296 0.302 0.299 0.149
6.250.252 0.248 0.250 0.100
00.152 0.148 0.150  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Gene References into Functions
  1. The PRRSV vaccination induced higher levels of IL-10 expression in the first week and this may be why the CSFV vaccination is immunosuppressed. PMID: 27938685
  2. These data indicate that the type 2 porcine reproductive and respiratory syndrome virus N protein plays an important role in IL-10 induction and the N-N non-covalent domain is associated with this activity. PMID: 25614594
  3. The effects of semen, spermatozoa in extender, or extender alone on the expression of TGF-beta1, IL-10, and IL-6 in ovarian follicles are reported. PMID: 24020676
  4. Prostaglandin E2 potentiates mesenchymal stem cell-induced IL-10+IFN-gamma+CD4+ regulatory T cells to control transplant arteriosclerosis. PMID: 23359497
  5. Porcine reproductive and respiratory syndrome virus strain CH-1a could significantly up-regulate IL-10 production through p38 MAPK activation. PMID: 22909062
  6. Boar seminal plasma contained TGF- beta1 and IL-10 but with high individual variation. PMID: 22482797
  7. Changes in interleukin-10 mRNA expression are predictive for 9-day survival of pigs in an emergency preservation and resuscitation model. PMID: 20163907
  8. These results suggest that TNF-alpha and IL-10, but not IL-6, are involved in the late reparatory phases of the experimental disk lesion. PMID: 19605905
  9. The present study shows that elevated levels of serum CRP and IL-10 were associated with porcine circovirus type 2-infected piglets that subsequently developed severe PMWS.(postweaning multisystemic wasting syndrome) PMID: 16817761
  10. Subclinically porcine circovirus type 2 (PCV2)-infected pigs developed a transient PCV2-specific IL-10 response during the viremic phase of infection which coincided with the inversion of the IgM/IgG ratio. PMID: 17592737
  11. Twenty one polymorphisms in the IL10 gene have been found, including single nucleotide polymorphisms and insertion deletion polymorphisms. The chromosomal location has been mapped by FISH and radiation hybrid mapping. PMID: 18607788
  12. findings show that Brachyspira hyodysenteriae inoculation induced production of systemic levels of IL-1beta during the dysentery period and increased levels of IL-10 coincided with recovery from dysentery PMID: 18700003

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Subcellular Location Secreted
Protein Families IL-10 family
Database Links

KEGG: ssc:397106

STRING: 9823.ENSSSCP00000016594

UniGene: Ssc.148

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