Pig serum amyloid A,SAA ELISA Kit

Code CSB-E13309p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Alternative Names
SAA2; Serum amyloid A-2 protein
Abbreviation
SAA
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma
Detection Range
19.53 pg/mL-5000 pg/mL
Sensitivity
19.53 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 97  
Range % 92-103  
1:2000 Average % 95  
Range % 91-99  
1:4000 Average % 101  
Range % 98-105  
1:8000 Average % 95  
Range % 86-96  
Recovery
The recovery of pig SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 88-99  
EDTA plasma (n=4) 103 98-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
5000 0.204 0.206 0.205    
1250 0.463 0.471 0.467    
312.5 0.915 0.935 0.925    
78.1 1.429 1.462 1.446    
19.53 2.056 2.138 2.097    
0 2.515 2.687 2.601    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig SAA ELISA Kit was designed for the quantitative measurement of Pig SAA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 19.53 pg/mL-5000 pg/mL and the sensitivity is 19.53 pg/mL.

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Target Background

Function
(From Uniprot)
Major acute phase reactant.
Subcellular Location
Secreted.
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