Rabbit Bone Alkaline Phosphatase (BALP) ELISA Kit

Code CSB-E15042Rb
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. G1SLU6
Lead Time 3-5 working days
Abbreviation BALP
Target Name Bone Alkaline Phosphatase (BALP)
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 0.156 mIU/mL-10 mIU/mL
Sensitivity 0.039 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %88-101
1:2Average %88
Range %81-92
1:4Average %89
Range %85-94
1:8Average %98
Range %90-101
Recovery
The recovery of rabbit BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10395-108
EDTA plasma (n=4)9991-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
102.136 2.087 2.112 2.009
51.839 1.783 1.811 1.708
2.51.513 1.488 1.501 1.398
1.251.079 1.028 1.054 0.951
0.6250.752 0.722 0.737 0.634
0.3120.562 0.554 0.558 0.455
0.1560.335 0.327 0.331 0.228
00.102 0.104 0.103
References
Still Have Questions? Leave a Message or Start an on-line Chat

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.