Rabbit Bone morphogenetic protein 2(BMP2) ELISA kit

Instructions
Code CSB-EL002736RB
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name bone morphogenetic protein 2
Alternative Names BMP2 ELISA Kit; BMP-2Bone morphogenetic protein 2 ELISA Kit; BMP-2 ELISA Kit
Abbreviation BMP2
Uniprot No. O46564
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BMP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:2 Average % 87
Range % 80-94
1:4 Average % 90
Range % 85-99
1:8 Average % 95
Range % 85-103
1:10 Average % 95
Range % 84-102
Recovery
The recovery of rabbit BMP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 82-100
EDTA plasma (n=4) 97 88-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
100 2.136 2.177 2.157 2.043
50 1.612 1.628 1.620 1.506
25 1.221 1.239 1.230 1.116
12.5 0.852 0.865 0.859 0.745
6.25 0.530 0.516 0.523 0.409
3.12 0.351 0.365 0.358 0.244
1.56 0.239 0.246 0.243 0.129
0 0.112 0.115 0.114  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.
Gene References into Functions
  1. The transfecting capability of a BMP-2 specific vector is examined and supports the idea that BMP-2 might diminish osseointegration and implant fixation. PMID: 21762501
  2. While BMP2 expression begins at (pregastrulation) stage 1 in the hypoblast, BMP4 expression commences--distinctly delayed compared to the mouse--diffusely at (pregastrulation) stage 2; from stage 3 onwards. PMID: 21881976
  3. Maxillary sinus floor elevation using BMP-2 gene-modified bone marrow stromal cells and TCP in rabbits PMID: 21584647
  4. Data show that BMP-2, BMP-4, and BMP-7, noggin, and chordin were colocalized in rimming osteoblasts, osteoclasts, and chondrocytes. PMID: 21099737
  5. BMP2 is not suitable to regenerate osteochondral lesions completely PMID: 19185914

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Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

KEGG: ocu:100009349

STRING: 9986.ENSOCUP00000010036

UniGene: Ocu.2192

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