Rabbit Epidermal growth factor,EGF ELISA Kit

Code CSB-E06909Rb
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Epidermal growth factor,EGF
Abbreviation
EGF
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.5 ng/mL-8 ng/mL
Sensitivity
0.25 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %111
Range %100-115
1:2Average %92
Range %84-97
1:4Average %100
Range %90-104
1:8Average %100
Range %93-104
Recovery
The recovery of rabbit EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9287-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
80.147 0.141 0.144
40.311 0.322 0.317
20.501 0.534 0.518
10.926 0.902 0.914
0.51.932 1.966 1.949
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit EGF ELISA Kit was designed for the quantitative measurement of Rabbit EGF protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.5 ng/mL-8 ng/mL and the sensitivity is 0.25 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

 Q&A
Q:

Can I check the ingredients of Stop solution for CSB-E06909Rb.
Please also advise if the standard is derived from CHO or E.coli.

A:
Thanks for your inquiry. Standard: CHO-derived recombinant protein
Stop solution: 4N sulfuric acid
Q:

Can you please also tell me if the standard is bacurovirus derived? We don't need the sequence, just the source.

A:
Thanks for your inquiry.The standard is not bacurovirus derived. It is CHO-derived recombinant protein.
Q:

I'd like to know the necessary sample volume for this ELISA kit.

A:
Thanks for your inquiry.The sample loading volume is 50ul per well.
CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1