Rabbit Epidermal growth factor,EGF ELISA Kit

Instructions
Code CSB-E06909Rb
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Epidermal growth factor,EGF
Abbreviation EGF
Uniprot No. G1TBJ6
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.5 ng/mL-8 ng/mL
Sensitivity 0.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %111
Range %100-115
1:2Average %92
Range %84-97
1:4Average %100
Range %90-104
1:8Average %100
Range %93-104
Recovery
The recovery of rabbit EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9287-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
80.147 0.141 0.144
40.311 0.322 0.317
20.501 0.534 0.518
10.926 0.902 0.914
0.51.932 1.966 1.949
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

Can I check the ingredients of Stop solution for CSB-E06909Rb.
Please also advise if the standard is derived from CHO or E.coli.

A:
Thanks for your inquiry. Standard: CHO-derived recombinant protein
Stop solution: 4N sulfuric acid
Q:

Can you please also tell me if the standard is bacurovirus derived? We don't need the sequence, just the source.

A:
Thanks for your inquiry.The standard is not bacurovirus derived. It is CHO-derived recombinant protein.
Q:

I'd like to know the necessary sample volume for this ELISA kit.

A:
Thanks for your inquiry.The sample loading volume is 50ul per well.

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  Email: support@cusabio.com
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