Rabbit Growth Hormone(GH) ELISA Kit

Code CSB-E15021Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
growth hormone 1
Alternative Names
GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
Abbreviation
GH1
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 ng/mL-100 ng/mL
Sensitivity
6.25 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 89    
Range % 82-97    
1:2 Average % 95    
Range % 89-100    
1:4 Average % 90    
Range % 85-100    
1:8 Average % 92    
Range % 89-102    
Recovery
The recovery of rabbit GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 96 90-110    
EDTA plasma (n=4) 95 89-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average      
100 0.169 0.171 0.170      
50 0.270 0.276 0.273      
25 0.496 0.488 0.492      
12.5 1.033 1.051 1.042      
6.25 2.062 2.053 2.058      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days
Description

This Rabbit GH1 ELISA Kit was designed for the quantitative measurement of Rabbit GH1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-100 ng/mL and the sensitivity is 6.25 ng/mL.

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Target Background

Function
(From Uniprot)
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Subcellular Location
Secreted.
Protein Families
Somatotropin/prolactin family
Database Links
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7505 Fannin St Ste 610-322 Houston, TX 77054, USA
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