Rabbit Interleukin 10,IL-10 ELISA Kit

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Rabbit Interleukin 10,IL-10 ELISA Kit

Name: Rabbit Interleukin 10,IL-10 ELISA Kit
Brand: CUSABIO
SKU: CSB-E06897Rb

^Instructions

Code	CSB-E06897Rb
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Target Name

interleukin 10

Alternative Names

IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit

Abbreviation

IL10

Uniprot No.

Q9TSJ4

Species

Oryctolagus cuniculus (Rabbit)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-98
1:2	Average %	96
	Range %	91-105
1:4	Average %	97
	Range %	92-110
1:8	Average %	93
	Range %	86-100

Recovery

The recovery of rabbit IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	89	80-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.627	2.549	2.588	2.461
1000	1.954	1.799	1.877	1.750
500	1.004	0.987	0.996	0.869
250	0.599	0.554	0.577	0.450
125	0.369	0.361	0.365	0.238
62.5	0.274	0.264	0.269	0.142
31.25	0.177	0.171	0.174	0.047
0	0.132	0.122	0.127

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Mesenchymal stem cells significantly improved treatment effects of Linezolid on severe pneumonia in a rabbit model Kong D, et al,Bioscience Reports,2019
Evaluation of the protective effects of a nanogel-based vaccine against rabbit hepatitis E virus Byung-JooPark, et al,Vaccine,2019
Poly(Lactic Acid) Blends with Poly(Trimethylene Carbonate) as Biodegradable Medical Adhesive Material Zhang S.et al,Int. J. Mol. Sci,2017
Clinical Features of Chronic Trypanosomosis in Rabbits Experimentally Infected with the Malaysian Isolate ofTrypanosoma evansi Yasameen et al,IOSR-JAVS,2015

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Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location	Secreted
Protein Families	IL-10 family
Database Links	STRING: 9986.ENSOCUP00000019534 UniGene: Ocu.1796

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Related pathways

Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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