Code | CSB-E13689Rb |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
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Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 96 | ||||
Range % | 91-103 | |||||
1:2 | Average % | 87 | ||||
Range % | 84-90 | |||||
1:4 | Average % | 95 | ||||
Range % | 89-99 | |||||
1:8 | Average % | 101 | ||||
Range % | 98-105 |
The recovery of rabbit MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 89 | 85-94 | ||||
EDTA plasma (n=4) | 93 | 89-97 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||
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Myeloperoxidase (MPO) is a heme-containing peroxidase enzyme primarily found in neutrophils and monocytes, where it plays an important role in the innate immune response. Upon activation, MPO catalyzes the formation of hypochlorous acid and other reactive oxygen species that contribute to antimicrobial defense mechanisms. Elevated MPO levels are associated with various inflammatory conditions, cardiovascular disease, and oxidative stress. This makes it a valuable biomarker for studying immune system activation and inflammatory processes in research applications.
The Rabbit Myeloperoxidase ELISA kit (CSB-E13689Rb) is designed for quantitative measurement of MPO in Oryctolagus cuniculus samples. This competitive assay accommodates serum, plasma, cell culture supernatants, and tissue homogenates with a detection range of 1.25-20 ng/mL and sensitivity of 1.25 ng/mL. The assay requires 50-100 μL sample volume, completes within 1-5 hours, and uses detection at 450 nm wavelength for reliable quantification of rabbit myeloperoxidase levels.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research investigating oxidative stress and inflammatory responses across multiple biological systems. Studies have applied the kit to measure myeloperoxidase activity in both serum and tissue samples as part of biochemical analyses. The kit supports research examining the relationship between inflammatory markers and tissue damage in various experimental models.
• Oxidative stress research: Measurement of myeloperoxidase activity alongside other oxidative markers such as malondialdehyde in serum samples to evaluate systemic oxidative burden
• Inflammatory biomarker studies: Quantification of myeloperoxidase in serum and tissue samples as part of multi-parameter inflammatory profiling that includes cytokines and other inflammatory mediators
• Tissue damage analysis: Measurement of myeloperoxidase levels in spinal cord tissue as part of biochemical evaluations examining inflammatory responses and tissue injury
• Comparative biomarker analysis: Integration of myeloperoxidase measurements with other inflammatory and oxidative stress markers to provide thorough analysis of biological responses in experimental studies
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