Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

Code CSB-E08524Rb
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation VEGF
Target Name Vascular Endothelial cell Growth Factor,VEGF
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-95  
1:2 Average % 100  
Range % 91-106  
1:4 Average % 94  
Range % 92-105  
1:8 Average % 90  
Range % 86-98  
Recovery
The recovery of rabbit VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 97 91-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.538 2.540 2.539 2.403  
50 2.012 2.022 2.017 1.881  
25 1.137 1.130 1.134 0.998  
12.5 0.602 0.651 0.627 0.491  
6.25 0.375 0.378 0.377 0.241  
3.12 0.279 0.285 0.282 0.146  
1.56 0.168 0.176 0.172 0.036  
0 0.131 0.141 0.136    
References
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Q&A and Customer Reviews

Q&A
Q:

Please provide the following information regarding Rabbit Vascular Endothelial cell Growth Factor, VEGF ELISA Kit (CSB-E08524Rb)
a. Can this kit detect human VEGF?

A:
Thanks for your inquiry.
About Vascular Endothelial cell Growth Factor,there is VEGFA~D for human while there is only VEGF for rabbit.
CSB-E08524Rb This kit is designed according to: https://www.uniprot.org/uniprot/Q866G4.
It detects VEGF and it can not test human samples.
Pls let me know if you have any further questions. Thank you.
Customer Reviews

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