Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

Code CSB-E08524Rb
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Vascular Endothelial cell Growth Factor,VEGF
Abbreviation
VEGF
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 pg/mL-100 pg/mL
Sensitivity
0.39 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-95  
1:2 Average % 100  
Range % 91-106  
1:4 Average % 94  
Range % 92-105  
1:8 Average % 90  
Range % 86-98  
Recovery
The recovery of rabbit VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 97 91-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.538 2.540 2.539 2.403  
50 2.012 2.022 2.017 1.881  
25 1.137 1.130 1.134 0.998  
12.5 0.602 0.651 0.627 0.491  
6.25 0.375 0.378 0.377 0.241  
3.12 0.279 0.285 0.282 0.146  
1.56 0.168 0.176 0.172 0.036  
0 0.131 0.141 0.136    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit VEGF ELISA Kit was designed for the quantitative measurement of Rabbit VEGF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

Citations

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 Q&A
Q:

Please provide the following information regarding Rabbit Vascular Endothelial cell Growth Factor, VEGF ELISA Kit (CSB-E08524Rb)
a. Can this kit detect human VEGF?

A:
Thanks for your inquiry.
About Vascular Endothelial cell Growth Factor,there is VEGFA~D for human while there is only VEGF for rabbit.
CSB-E08524Rb This kit is designed according to: https://www.uniprot.org/uniprot/Q866G4.
It detects VEGF and it can not test human samples.
Pls let me know if you have any further questions. Thank you.
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