Target Name
cross linked C-telopeptide of type Ⅱ collagen
Alternative Names
cross linked C-telopeptide of type 2 collagen (CTX-2),cross linked C-telopeptide of type Ⅱ collagen (CTX-Ⅱ)
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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1:1 |
Average % |
95 |
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Range % |
89-103 |
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1:2 |
Average % |
90 |
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Range % |
86-99 |
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1:4 |
Average % |
95 |
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Range % |
90-102 |
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1:8 |
Average % |
92 |
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Range % |
83-99 |
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Recovery
The recovery of rabbit CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
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Serum (n=5) |
97 |
92-103 |
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EDTA plasma (n=4) |
95 |
89-106 |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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40 |
2.547 |
2.652 |
2.600 |
2.462 |
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20 |
2.037 |
1.962 |
2.000 |
1.862 |
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10 |
1.449 |
1.376 |
1.413 |
1.275 |
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5 |
0.902 |
0.866 |
0.884 |
0.746 |
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2.5 |
0.514 |
0.526 |
0.520 |
0.382 |
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1.25 |
0.332 |
0.347 |
0.340 |
0.202 |
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0.625 |
0.246 |
0.253 |
0.250 |
0.112 |
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0 |
0.139 |
0.136 |
0.138 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Rabbit CTX-Ⅱ ELISA Kit was designed for the quantitative measurement of Rabbit CTX-Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.