Rabbit cross linked C-telopeptide of type Ⅱ collagen (CTX-Ⅱ) ELISA Kit

Code CSB-E16213Rb
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
cross linked C-telopeptide of type Ⅱ collagen
Alternative Names
cross linked C-telopeptide of type 2 collagen (CTX-2),cross linked C-telopeptide of type Ⅱ collagen (CTX-Ⅱ)
Abbreviation
CTX-Ⅱ
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 89-103  
1:2 Average % 90  
Range % 86-99  
1:4 Average % 95  
Range % 90-102  
1:8 Average % 92  
Range % 83-99  
Recovery
The recovery of rabbit CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-103  
EDTA plasma (n=4) 95 89-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.547 2.652 2.600 2.462  
20 2.037 1.962 2.000 1.862  
10 1.449 1.376 1.413 1.275  
5 0.902 0.866 0.884 0.746  
2.5 0.514 0.526 0.520 0.382  
1.25 0.332 0.347 0.340 0.202  
0.625 0.246 0.253 0.250 0.112  
0 0.139 0.136 0.138    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit CTX-Ⅱ ELISA Kit was designed for the quantitative measurement of Rabbit CTX-Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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