Rabbit prothrombin fragment 1+2 (F1+2) ELISA kit

Code CSB-EQ027672RB
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
prothrombin fragment 1+2 (F1+2)
Abbreviation
F1+2
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 pmol/mL-20 pmol/mL
Sensitivity
0.078 pmol/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-88  
1:2 Average % 98  
Range % 92-104  
1:4 Average % 97  
Range % 93-101  
1:8 Average % 87  
Range % 82-95  
Recovery
The recovery of rabbit F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 83-92  
EDTA plasma (n=4) 92 85-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pmol/ml OD1 OD2 Average Corrected  
20 2.989 2.788 2.889 2.786  
10 2.535 2.372 2.454 2.351  
5 1.781 1.815 1.798 1.695  
2.5 1.002 1.012 1.007 0.904  
1.25 0.485 0.506 0.496 0.393  
0.625 0.301 0.311 0.306 0.203  
0.312 0.178 0.175 0.177 0.074  
0 0.103 0.102 0.103    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit F1+2 ELISA Kit was designed for the quantitative measurement of Rabbit F1+2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 pmol/mL-20 pmol/mL and the sensitivity is 0.078 pmol/mL.

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