Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit

Instructions
Code CSB-E12730r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name orosomucoid 1
Alternative Names Orm1Alpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
Abbreviation ORM1
Uniprot No. P02764
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, urine, cell culture supernates, tissue homogenates
Detection Range 3.125 ng/mL-800 ng/mL
Sensitivity 3.125 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 95
Range % 85-105
1:2000 Average % 89
Range % 81-98
1:4000 Average % 90
Range % 82-97
1:8000 Average % 95
Range % 83-98
Recovery
The recovery of rat α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-102
EDTA plasma (n=4) 95 90-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
800 0.213 0.217 0.215
200 0.459 0.453 0.456
50 0.851 0.859 0.855
12.5 1.226 1.246 1.236
3.125 1.727 1.740 1.734
0 1.935 1.958 1.947
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
Gene References into Functions
  1. fatigue upregulates the level of ORM1, which in turn functions as an anti-fatigue protein to enhance muscle endurance via the CCR5 pathway. PMID: 26740279
  2. context-dependent nature of ORM1 angioregulatory function is further demonstrated in the CAM assay where ORM1 stimulates developmental angiogenesis without exerting any inhibitory activity PMID: 22916107
  3. alpha-1 acid glycoprotein expression is increased by retinoids at the transcriptional level through two distinct DR1 retinoic acid responsive elements PMID: 15157739
  4. Surprisingly, AGP-over-expressing mice were not protected against renal ischemia-reperfusion injury. PMID: 15502707
  5. Orm1 gene expression highly induced in cardiomyocytes overexpressing mineralocorticoid receptor and treated with aldosterone. PMID: 17234708

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Subcellular Location Secreted.
Protein Families Calycin superfamily, Lipocalin family
Database Links

KEGG: rno:24614

STRING: 10116.ENSRNOP00000010454

UniGene: Rn.10295

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