Rat 5-Hydroxytryptamine,5HT ELISA Kit

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Name: Rat 5-Hydroxytryptamine,5HT ELISA Kit
Brand: CUSABIO
SKU: CSB-E08364r

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Code	CSB-E08364r
Size	96T,5×96T,10×96T
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Target Name

5-Hydroxytryptamine,5HT

Abbreviation

5HT

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell lysates, tissue homogenates

Detection Range

0.8 ng/mL-160 ng/mL

Sensitivity

0.4 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	95
	Range %	92-99
1:4	Average %	97
	Range %	89-103
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of rat 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-102
EDTA plasma (n=4)	96	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
160	0.069	0.074	0.072
40	0.181	0.195	0.188
12.8	0.426	0.439	0.433
3.2	0.802	0.855	0.829
0.8	1.155	1.231	1.193
0	2.158	2.227	2.193

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Oral administration of 5-Hydroxytryptophan aggravated periodontitis-induced alveolar bone loss in rats Xianxian Li et al,Archives of Oral Biology,2015
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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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