Rat 5-Hydroxytryptamine,5HT ELISA Kit

Instructions
Code CSB-E08364r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name 5-Hydroxytryptamine,5HT
Abbreviation 5HT
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell lysates, tissue homogenates
Detection Range 0.8 ng/mL-160 ng/mL
Sensitivity 0.4 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 89-98  
1:2 Average % 95  
Range % 92-99  
1:4 Average % 97  
Range % 89-103  
1:8 Average % 95  
Range % 90-100  
Recovery
The recovery of rat 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 91-102  
EDTA plasma (n=4) 96 92-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
160 0.069 0.074 0.072    
40 0.181 0.195 0.188    
12.8 0.426 0.439 0.433    
3.2 0.802 0.855 0.829    
0.8 1.155 1.231 1.193    
0 2.158 2.227 2.193    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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