Rat 5-Hydroxytryptamine,5HT ELISA Kit

The Rat 5-Hydroxytryptamine (5HT) ELISA kit is used to detect and quantify the concentrations of INS in serum, plasma, cell lysates, and tissue homogenates. This kit exclusively recognizes rat 5HT proteins. This ELISA kit has been confirmed to have high sensitivity, strong specificity, premium precision, high recovery, good linearity, and lot-to-lot consistency. See the instructions for more details.

Standards or samples are added to the appropriate microtiter plate wells pre-coated with goat-anti-rabbit 5HT antibody. HRP-conjugated 5HT and anti-rat 5HT antibody are pipped into the wells, where 5HT in the sample or standard competes with the HRP labeled 5HT for limited binding sites on the anti-rat 5HT antibody. These Ag-Ab complexes are captured by the antibody immobilized on the plate. The solution color turns blue after the addition of substrate A and B. The color reaction is immediately terminated after the addition of the stop solution. And the color changes from blue to yellow. The color develops in opposition to the amount of 5HT in the sample, and the intensity of the color is measured by a Microplate reader at 450 nm.

5-HT, also called serotonin, is a monoamine neurotransmitter that plays a critical role in the central nervous system (CNS). 5-HT effects are mediated through its interaction with 5-HT receptors (5-HTRs). 5-HT regulates mood stability, pain tolerance, and sleep patterns. It is also involved in the modulation of individual behaviors that organize social group dynamics.