| Target Name |
5-Hydroxytryptamine,5HT |
| Abbreviation |
5HT |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell lysates, tissue homogenates |
| Detection Range |
0.8 ng/mL-160 ng/mL |
| Sensitivity |
0.4 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience
|
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
94 |
|
| Range % |
89-98 |
|
| 1:2 |
Average % |
95 |
|
| Range % |
92-99 |
|
| 1:4 |
Average % |
97 |
|
| Range % |
89-103 |
|
| 1:8 |
Average % |
95 |
|
| Range % |
90-100 |
|
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| Recovery |
| The recovery of rat 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
91-102 |
|
| EDTA plasma (n=4) |
96 |
92-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml |
OD1 |
OD2 |
Average |
|
|
| 160 |
0.069 |
0.074 |
0.072 |
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| 40 |
0.181 |
0.195 |
0.188 |
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| 12.8 |
0.426 |
0.439 |
0.433 |
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|
| 3.2 |
0.802 |
0.855 |
0.829 |
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| 0.8 |
1.155 |
1.231 |
1.193 |
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|
| 0 |
2.158 |
2.227 |
2.193 |
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Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
