Rat Acetylcholinesterase,AChE ELISA Kit

Code CSB-E11304r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P37136
Lead Time 3-5 working days
Abbreviation ACHE
Protein Biological Process 1 Neurobiology
Target Name acetylcholinesterase (Yt blood group)
Alias ARACHE, N-ACHE, YT, OTTHUMP00000211347|OTTHUMP00000211349|OTTHUMP00000211356|acetylcholinesterase|apoptosis-related acetylcholinesterase
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Neurotransmitter degradation
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.
HGNC 108
RGD 69313
MGI 87876
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %92
Range %88-95
1:200Average %96
Range %90-101
1:400Average %94
Range %90-97
1:800Average %99
Range %95-103
Recovery
The recovery of rat AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-99
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.361 2.384 2.373 2.232
2501.636 1.658 1.647 1.506
1251.044 1.057 1.051 0.910
62.50.610 0.599 0.605 0.464
31.20.413 0.404 0.409 0.268
15.60.305 0.297 0.301 0.160
7.80.237 0.228 0.233 0.092
00.142 0.140 0.141  
References
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Function Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Subcellular Location Cell junction, synapse, Secreted, Cell membrane, Peripheral membrane protein, SUBCELLULAR LOCATION: Isoform H: Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side
Protein Families Type-B carboxylesterase/lipase family
Tissue Specificity Has been found in central nervous system and muscle. Found in embryonic liver and spleen but not in adult liver.
Database Links

KEGG: rno:83817

UniGene: Rn.105879

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