Rat Acylated Ghrelin (AG) ELISA Kit

Code CSB-E13167r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Abbreviation
AG
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %87-101
1:2Average %86
Range %80-92
1:4Average %93
Range %90-95
1:8Average %95
Range %92-98
Recovery
The recovery of rat AG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10095-104
EDTA plasma (n=4)9795-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.690 2.699 2.695 2.502
2001.885 1.899 1.892 1.699
1001.162 1.198 1.180 0.987
500.798 0.760 0.779 0.586
250.581 0.535 0.558 0.365
12.50.388 0.376 0.382 0.189
6.250.301 0.321 0.311 0.118
00.191 0.195 0.193  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Acylated Ghrelin (AG) ELISA Kit is a cutting-edge solution for the accurate and efficient detection and quantification of Acylated Ghrelin in rat samples. Acylated Ghrelin is a hormone produced in the stomach that has been implicated in a variety of physiological processes. This ELISA kit is designed to detect and measure Acylated Ghrelin in rat serum, plasma, and tissue homogenates, with a detection range of 6.25 pg/mL-400 pg/mL and a sensitivity of 1.56 pg/mL. This provides precise and accurate results, allowing for the measurement of even the smallest amounts of Acylated Ghrelin in your samples.

The assay time ranges from 1-5 hours, and requires only a small sample volume of 50-100ul, making our ELISA kit a quick and efficient solution for your research needs. The detection wavelength of 450 nm provides clear and reliable readings.

It operates using a sandwich assay principle, ensuring accurate and precise measurements of Acylated Ghrelin in your samples. The kit's high sensitivity and specificity make it an essential tool for researchers investigating the role of Acylated Ghrelin in various physiological processes.

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