Rat Alpha-fetoprotein,AFP ELISA Kit

Instructions
Code CSB-E08281r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name alpha-fetoprotein
Alternative Names AfpAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
Abbreviation AFP
Uniprot No. P02773
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:20Average %85
Range %81-90
1:40Average %89
Range %82-94
1:80Average %101
Range %96-108
1:160Average %94
Range %89-99
Recovery
The recovery of rat AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9086-94
EDTA plasma (n=4)9793-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.701 2.631 2.666 2.478
5002.239 2.212 2.226 2.038
2501.710 1.663 1.687 1.499
1251.097 1.174 1.136 0.948
62.50.651 0.695 0.673 0.485
31.20.427 0.459 0.443 0.255
15.60.337 0.317 0.327 0.139
00.192 0.184 0.188  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Binds estrogens, fatty acids and metals.
Subcellular Location Isoform 1: Secreted, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma.
Database Links

STRING: 10116.ENSRNOP00000003879

UniGene: Rn.9174

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