Code | CSB-E13885r |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat AT III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:200 | Average % | 104 | |||||
Range % | 100-112 | ||||||
1:400 | Average % | 84 | |||||
Range % | 80-88 | ||||||
1:800 | Average % | 90 | |||||
Range % | 85-94 | ||||||
1:1600 | Average % | 97 | |||||
Range % | 92-101 |
The recovery of rat AT III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 88 | 84-92 | |||||
EDTA plasma (n=4) | 93 | 89-100 | |||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Rat Antithrombin III (AT III) ELISA Kit is designed to measure Antithrombin-III (AT III) in rat serum, plasma, and tissue homogenates with remarkable accuracy and sensitivity.
With a detection range of 39 ng/mL-2500 ng/mL and a sensitivity of 9.75 ng/mL, this quantitative assay can detect even the smallest amount of AT III in your samples. The assay time is only 1-5 hours, and the required sample volume is just 50-100ul. The detection wavelength is 450 nm, ensuring reliable and precise results every time.
This kit is ideal for researchers investigating the role of AT III in cardiovascular diseases、anticoagulant system research. The sandwich measurement principle allows for accurate quantification of AT III in your samples, making it a valuable tool for any cardiovascular research project. This Rat Antithrombin III (AT III) ELISA Kit has been cited in 2 scientific publications, which speaks to its reliability and popularity within the research community.
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