Rat Antithrombin III(AT III)ELISA Kit

Code CSB-E13885r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Antithrombin III(AT III)
Abbreviation
AT III
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
39 ng/mL-2500 ng/mL
Sensitivity
9.75 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AT III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 104
Range % 100-112
1:400 Average % 84
Range % 80-88
1:800 Average % 90
Range % 85-94
1:1600 Average % 97
Range % 92-101
Recovery
The recovery of rat AT III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 84-92
EDTA plasma (n=4) 93 89-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
2500 2.466 2.312 2.389 2.251
1250 2.075 1.996 2.036 1.898
625 1.664 1.689 1.677 1.539
312.5 1.238 1.227 1.233 1.095
156 0.702 0.713 0.708 0.570
78 0.412 0.392 0.402 0.264
39 0.301 0.289 0.295 0.157
0 0.135 0.141 0.138  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Antithrombin III (AT III) ELISA Kit is designed to measure Antithrombin-III (AT III) in rat serum, plasma, and tissue homogenates with remarkable accuracy and sensitivity.

With a detection range of 39 ng/mL-2500 ng/mL and a sensitivity of 9.75 ng/mL, this quantitative assay can detect even the smallest amount of AT III in your samples. The assay time is only 1-5 hours, and the required sample volume is just 50-100ul. The detection wavelength is 450 nm, ensuring reliable and precise results every time.

This kit is ideal for researchers investigating the role of AT III in cardiovascular diseases、anticoagulant system research. The sandwich measurement principle allows for accurate quantification of AT III in your samples, making it a valuable tool for any cardiovascular research project. This Rat Antithrombin III (AT III) ELISA Kit has been cited in 2 scientific publications, which speaks to its reliability and popularity within the research community.

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