Rat Antithrombin III(AT III)ELISA Kit

Instructions
Code CSB-E13885r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Antithrombin III(AT III)
Abbreviation AT III
Uniprot No. Q5M7T5
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 39 ng/mL-2500 ng/mL
Sensitivity 9.75 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AT III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 104
Range % 100-112
1:400 Average % 84
Range % 80-88
1:800 Average % 90
Range % 85-94
1:1600 Average % 97
Range % 92-101
Recovery
The recovery of rat AT III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 84-92
EDTA plasma (n=4) 93 89-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
2500 2.466 2.312 2.389 2.251
1250 2.075 1.996 2.036 1.898
625 1.664 1.689 1.677 1.539
312.5 1.238 1.227 1.233 1.095
156 0.702 0.713 0.708 0.570
78 0.412 0.392 0.402 0.264
39 0.301 0.289 0.295 0.157
0 0.135 0.141 0.138  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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