Rat Apolipoprotein A-IV(APOA4) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

apolipoprotein A-IV

Alternative Names

Apoa4Apolipoprotein A-IV ELISA kit; Apo-AIV ELISA kit; ApoA-IV ELISA kit; Apolipoprotein A4 ELISA kit

Abbreviation

APOA4

Uniprot No.

P02651

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

9.38 ng/mL-600 ng/mL

Sensitivity

2.34 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat APOA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	93
	Range %	87-97
1:400	Average %	101
	Range %	97-105
1:800	Average %	92
	Range %	88-96
1:1600	Average %	95
	Range %	91-101

Recovery

The recovery of rat APOA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	89	83-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
600	2.567	2.615	2.591	2.454
300	1.934	2.078	2.006	1.869
150	1.324	1.397	1.361	1.224
75	0.831	0.822	0.827	0.690
37.5	0.581	0.574	0.578	0.441
18.75	0.334	0.323	0.329	0.192
9.38	0.261	0.268	0.265	0.128
0	0.136	0.137	0.137

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The Rat Apolipoprotein A-IV (APOA4) ELISA kit is a powerful tool for the quantitative detection of Apolipoprotein A-IV in serum, plasma, and tissue homogenates from Rattus norvegicus (Rat) samples.

This ELISA kit offers a wide detection range of 9.38 ng/mL to 600 ng/mL and a sensitivity of 2.34 ng/mL, enabling precise and accurate quantification of APOA4 in your samples. The assay time is short, ranging from 1 to 5 hours, and requires only a small sample volume of 50-100ul.

The assay principle is a sandwich method, where the APOA4 in the sample is captured by a coated antibody and detected by a biotinylated detection antibody, followed by a streptavidin-HRP conjugate, and finally substrate solution. The signal is detected at a wavelength of 450 nm.

This kit is suitable for research in various areas and is particularly useful for studies involving cancer and metabolism research. The Rat Apolipoprotein A-IV (APOA4) ELISA kit provides reliable and consistent results for your experiments.

Target Background

Function
(From Uniprot)

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.

Gene References into Functions

demonstrate a critical role of the PI3K/Akt pathway in apoA-IV's anorectic action in lean rats and suggest a defective PI3K pathway in the ventromedial hypothalamus is responsible for the impaired apoA-IV's anorectic action in the diet-induced obese animals PMID: 29037812
These results suggest that the elevated mRNA expression levels of Rbp2 and Apoa4 in the rats with SB contribute to the effective esterification and transport of vitamin A. PMID: 25585692
Compared to the rats in the control group, the rats in the HF group had the expression levels of 12 genes with significant changes, Agrp, Ghrl, and Nr3c1, Apoa4, Cntf, Ghr, IL-1beta, Ins1, Lepr,Sort, Adcyap1r1 and Adrb1. PMID: 22715380
Nuclear ERalpha is the primary mediator of E2's action on apo A-IV gene expression. PMID: 25051443
Data suggest that apoAIV in NTS (nucleus of the solitary tract) or/and peripheral Cck (cholecystokinin) require vagal Cck1r (cholecystokinin receptor 1) signaling to elicit satiation; high-fat diet reduces satiating capacity of these signals. PMID: 24564397
Octreotide intervention down-regulated expression of apoB mRNA and apoB48 protein, as well as MTP mRNA and proteins. ApoAIV mRNA was not significantly different between obese and octreotide-treated rats. PMID: 23911221
peripheral apo AIV requires an intact CCK system and vagal afferents to activate neurons in the hindbrain to reduce food intake PMID: 23027805
hypothalamic apo A-IV level and food intake were inversely related during the normal diurnal cycle as well as in the period of restricted feeding suggesting that hypothalamic apo A-IV is involved in the regulation of daily food intake. PMID: 15059955
Chronic consumption of a high fat diet significantly reduced apolipoprotein A-IV (apo A-IV) mRNA levels and response of apo A-IV gene expression to dietary lipids in the hypothalamus. PMID: 15082422
apo A-IV released from the intestinal mucosa during lipid absorption stimulates the release of endogenous CCK that activates CCK(1) receptors on vagal afferent nerve terminals initiating feedback inhibition of gastric motility. PMID: 15117731
data support a synergistic interaction between apo A-IV and melanocortins that reduces food intake by acting downstream of the arcuate PMID: 16166201
that apo A-IV, produced by neuronal cells iin brain, may exert its anorectic action by interacting with catabolic regulatory neuropeptides. PMID: 18577393
Intraperitoneally injected Apoa4 inhibited food intake and levels were measured in intestinal lymph. PMID: 19020287

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Subcellular Location

Secreted.

Protein Families

Apolipoprotein A1/A4/E family

Tissue Specificity

Secreted in plasma.

Database Links

KEGG: rno:25080

UniGene: Rn.15739

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Code	CSB-EL001916RA
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.