Rat Apolipoprotein A-IV(APOA4) ELISA kit

Instructions
Code CSB-EL001916RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein A-IV
Alternative Names Apoa4Apolipoprotein A-IV ELISA kit; Apo-AIV ELISA kit; ApoA-IV ELISA kit; Apolipoprotein A4 ELISA kit
Abbreviation APOA4
Uniprot No. P02651
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 9.38 ng/mL-600 ng/mL
Sensitivity 2.34 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat APOA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:200Average %93
Range %87-97
1:400Average %101
Range %97-105
1:800Average %92
Range %88-96
1:1600Average %95
Range %91-101
Recovery
The recovery of rat APOA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)8983-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
6002.567 2.615 2.591 2.454
3001.934 2.078 2.006 1.869
1501.324 1.397 1.361 1.224
750.831 0.822 0.827 0.690
37.50.581 0.574 0.578 0.441
18.750.334 0.323 0.329 0.192
9.380.261 0.268 0.265 0.128
00.136 0.137 0.137
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Gene References into Functions
  1. demonstrate a critical role of the PI3K/Akt pathway in apoA-IV's anorectic action in lean rats and suggest a defective PI3K pathway in the ventromedial hypothalamus is responsible for the impaired apoA-IV's anorectic action in the diet-induced obese animals PMID: 29037812
  2. These results suggest that the elevated mRNA expression levels of Rbp2 and Apoa4 in the rats with SB contribute to the effective esterification and transport of vitamin A. PMID: 25585692
  3. Compared to the rats in the control group, the rats in the HF group had the expression levels of 12 genes with significant changes, Agrp, Ghrl, and Nr3c1, Apoa4, Cntf, Ghr, IL-1beta, Ins1, Lepr,Sort, Adcyap1r1 and Adrb1. PMID: 22715380
  4. Nuclear ERalpha is the primary mediator of E2's action on apo A-IV gene expression. PMID: 25051443
  5. Data suggest that apoAIV in NTS (nucleus of the solitary tract) or/and peripheral Cck (cholecystokinin) require vagal Cck1r (cholecystokinin receptor 1) signaling to elicit satiation; high-fat diet reduces satiating capacity of these signals. PMID: 24564397
  6. Octreotide intervention down-regulated expression of apoB mRNA and apoB48 protein, as well as MTP mRNA and proteins. ApoAIV mRNA was not significantly different between obese and octreotide-treated rats. PMID: 23911221
  7. peripheral apo AIV requires an intact CCK system and vagal afferents to activate neurons in the hindbrain to reduce food intake PMID: 23027805
  8. hypothalamic apo A-IV level and food intake were inversely related during the normal diurnal cycle as well as in the period of restricted feeding suggesting that hypothalamic apo A-IV is involved in the regulation of daily food intake. PMID: 15059955
  9. Chronic consumption of a high fat diet significantly reduced apolipoprotein A-IV (apo A-IV) mRNA levels and response of apo A-IV gene expression to dietary lipids in the hypothalamus. PMID: 15082422
  10. apo A-IV released from the intestinal mucosa during lipid absorption stimulates the release of endogenous CCK that activates CCK(1) receptors on vagal afferent nerve terminals initiating feedback inhibition of gastric motility. PMID: 15117731
  11. data support a synergistic interaction between apo A-IV and melanocortins that reduces food intake by acting downstream of the arcuate PMID: 16166201
  12. that apo A-IV, produced by neuronal cells iin brain, may exert its anorectic action by interacting with catabolic regulatory neuropeptides. PMID: 18577393
  13. Intraperitoneally injected Apoa4 inhibited food intake and levels were measured in intestinal lymph. PMID: 19020287

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Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Secreted in plasma.
Database Links

KEGG: rno:25080

UniGene: Rn.15739

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