Rat Apolipoprotein M(APOM) ELISA kit

Code CSB-EL001947RA
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
apolipoprotein M
Alternative Names
ApomApolipoprotein M ELISA kit; Apo-M ELISA kit; ApoM ELISA kit; Protein Px ELISA kit
Abbreviation
APOM
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 96
Range % 92-100
1:400 Average % 90
Range % 85-96
1:800 Average % 90
Range % 88-94
1:1600 Average % 100
Range % 96-105
Recovery
The recovery of rat APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-94
EDTA plasma (n=4) 95 90-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
100 2.454 2.460 2.457 2.319
50 2.045 2.032 2.039 1.901
25 1.411 1.482 1.447 1.309
12.5 0.831 0.901 0.866 0.728
6.25 0.631 0.625 0.628 0.490
3.12 0.379 0.408 0.394 0.256
1.56 0.277 0.272 0.275 0.137
0 0.135 0.140 0.138  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat APOM ELISA Kit was designed for the quantitative measurement of Rat APOM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

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Target Background

Function
(From Uniprot)
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
Gene References into Functions
  1. Hyperglycemia-induced downregulation of apolipoprotein M expression is not via the hexosamine pathway. PMID: 26377577
  2. ApoM occurrence in urine may be considered to be a biomarker of acute renal injury. PMID: 23953565
  3. ApoM levels and expression in rats rendered hyperglycemic by short-term glucose infusion, was investigated. PMID: 17556016
Subcellular Location
Secreted. Note=Associated with HDL.
Protein Families
Calycin superfamily, Lipocalin family
Tissue Specificity
Expressed by the liver; secreted in plasma.
Database Links

KEGG: rno:55939

STRING: 10116.ENSRNOP00000001126

UniGene: Rn.262

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