Rat Arachidonate 5-lipoxygenase (Alox5) ELISA kit

Instructions
Code CSB-E16982r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Arachidonate 5-lipoxygenase (Alox5)
Alternative Names Alox5Arachidonate 5-lipoxygenase ELISA Kit; 5-LO ELISA Kit; 5-lipoxygenase ELISA Kit; EC 1.13.11.34 ELISA Kit
Abbreviation Alox5
Uniprot No. P12527
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Alox5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 92-100
1:2 Average % 102
Range % 95-106
1:4 Average % 90
Range % 83-94
1:8 Average % 93
Range % 88-97
Recovery
The recovery of rat Alox5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 84-95
EDTA plasma (n=4) 100 92-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.817 2.743 2.780 2.666
5 2.369 2.304 2.337 2.223
2.5 1.574 1.521 1.548 1.434
1.25 0.825 0.789 0.807 0.693
0.625 0.418 0.402 0.410 0.296
0.312 0.234 0.228 0.231 0.117
0.156 0.161 0.165 0.163 0.049
0 0.113 0.115 0.114  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Gene References into Functions
  1. 5-LOX plays an important role in activating apoptosis and repressing neutrophils activation during acute necrotizing pancreatitis. PMID: 29421656
  2. Our results indicate that 5-lipoxygenase activation is mechanistically involved in chronic hepatic injury in a rat model of chronic aluminum overload exposure and that the 5-LO signaling pathway, which associated with inflammation and oxidative stress, is a potential therapeutic target for chronic non-infection liver diseases PMID: 27368151
  3. Results support 5-lipoxygenase involvement in the effect of glutoxim and molixan on intracellular Ca(2+) concentration in macrophages and indicate the inadvisability of a combined use of drugs glutoxim and molixan and antiasthmatic agent zileuton PMID: 27599517
  4. 12/15-LO-derived oxidized lipids regulate histone modifications associated with profibrotic gene expression in MCs, and 12/15-LO can mediate similar actions of TGF-beta1 and diabetes. PMID: 26492974
  5. Alox5 affects the osteogenic and adipogenic abilities of iPSCs in vivo and the effect of Cox2 inhibition in this system. PMID: 24906289
  6. 5-Lipoxygenase and 5-LO activating protein (FLAP) colocalize in the interstitium of the renal medulla following trauma and hemorrhagic shock.. PMID: 24747451
  7. Urotensin II increased aortic adventitial fibroblast expression of the 5-LO mRNA/protein by NF-kappaB activation through p38 MAPK and ERK pathways. PMID: 23053343
  8. 5-lipoxygenase expression can be considered as a critical event in controlling the process of mammary tumor development. PMID: 22213124
  9. Cochinchina momordica seed extracts exerted significantly protective effect against cysteamine-induced duodenal ulcer by 5-lipoxygenase inhibition. PMID: 22486866
  10. Zileuton can reduce rotenone-induced 5-LOX activation and cell injury in PC12 cells. PMID: 21488210
  11. Cochinchina momordica seed extract is a gastroprotective agent against acute gastric mucosal damage by suppressing proinflammatory cytokines, downregulating cPLA(2), 5-LOX, and increasing the synthesis of mucus. PMID: 19130224
  12. Our results confirm the key role of both COX-2 and 5-LOX in the inflammatory response to acute pancreatitis. PMID: 20977452
  13. ALOX5 gene silencing decreases the frequency of necrotic cell death after anoxia-reoxygenation of cultured cardiomyocytes as well as reduces heart infarct area size at ischemia-reperfusion in vivo. PMID: 20011686
  14. prolonged LPS treatment modifies activity in alveolar macrophages PMID: 12385890
  15. hyperoxia increases LTB(4) output, as well as protein levels of 5-LO and FLAP, in newborn rat lungs during early postnatal life. PMID: 12490039
  16. Enzyme is down-regulated and leukotriene production reduced by prolonged exposure to lipopolysaccharide. PMID: 12664571
  17. Role of 5-Lipoxygenase in angiotensin II-induced NAD(P)H-oxidase activation. PMID: 12914802
  18. Plays critical role in progression of pulmonary hypertension. Detrimental effect of 5-lipoxygenase is manifest only in setting of pulmonary vascular endothelial cell dysfunction. PMID: 14726295
  19. arachidonic acid metabolism through LOX activity, is a major source of extracellular O(2)(*-) release in skeletal muscle. PMID: 15107407
  20. iNOS regulates alveolar macrophage 5-lipoxygenase metabolism following endotoxin exposure. PMID: 15371096
  21. 5-lipoxygenase nuclear import is regulated by multiple nuclear localization sequences PMID: 15479450
  22. Basal iPLA2 activity, through the generation of 5-LO metabolites, regulates AMPA receptor phosphorylation of GluR1 subunits, an effect that might selectively influence the number of membrane receptors in area CA1 of the hippocampus. PMID: 15630695
  23. Activation of the 5-lipoxygenase pathway previously reported in streptozotocin-induced diabetic rats could be explained by an augmented sensitivity to cysteinyl leukotrienes of the diabetic aorta. PMID: 15789618
  24. Our results suggest that Nordy was capable of reducing the level of malignancy of glioma cells. PMID: 16516855
  25. From these results, we conclude that OGD can activate 5-LOX in primary rat cortical neurons, and that this activation may be partly mediated via activating NMDA receptor. PMID: 16606359
  26. Data show that the rat air pouch model is a reliable and useful tool for evaluating in vivo efficacy of 5-LOX inhibitors. PMID: 18295198
  27. atorvastatin and pioglitazone induced an interaction between 5LO and COX2 in the cytosolic fraction of myocardial cells PMID: 18714024
  28. nuclear import, which is regulated by phosphorylation on Ser-523, to determine the subcellular distribution of 5-LO, which in turn regulates leukotriene biosynthesis. PMID: 18978352
  29. Lysophosphatidylcholine caused the enhancement of oxidative stress in aged aorta through reactive species generation by an activated 5-lipoxygenase pathway. PMID: 19226207

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Subcellular Location Cytoplasm, Nucleus matrix, Nucleus membrane, Peripheral membrane protein
Protein Families Lipoxygenase family
Database Links

KEGG: rno:25290

STRING: 10116.ENSRNOP00000017633

UniGene: Rn.9662

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