Rat Atrial Natriuretic Peptide,ANP ELISA Kit

Instructions
Code CSB-E12982r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name natriuretic peptide precursor A
Alternative Names NppaNatriuretic peptides A ELISA Kit; Prepronatriodilatin) [Cleaved into: Atrial natriuretic factor ELISA Kit; ANF ELISA Kit; Atrial natriuretic peptide ELISA Kit; ANP); Auriculin-B; Auriculin-A; Atriopeptin-1 ELISA Kit; Atriopeptin I); Atriopeptin-2 ELISA Kit; Atriopeptin II); Atriopeptin-3 ELISA Kit; Atriopeptin III)] ELISA Kit
Abbreviation NPPA
Uniprot No. P01161
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %97
Range %92-105
1:10Average %94
Range %89-97
1:20Average %91
Range %87-95
1:40Average %92
Range %86-96
Recovery
The recovery of rat ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8782-95
EDTA plasma (n=4)9690-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.494 2.595 2.545 2.423
2501.722 1.823 1.773 1.651
1251.110 1.131 1.121 0.999
62.50.615 0.646 0.631 0.509
31.20.407 0.396 0.402 0.280
15.60.307 0.296 0.302 0.180
7.80.199 0.196 0.198 0.076
00.123 0.121 0.122  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

Function
(From Uniprot)
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 (By similarity).
Gene References into Functions
  1. we suggest that the order of secretagogue effect of ET family on ANP secretion was ET-1>/=ET-2>>ET-3>s6C and ET-1-induced atrial natriuretic peptide secretion negatively regulates the pressor effect of ET-1. PMID: 27208702
  2. Despite preserved LV structure and function, treatment with GYY increased the levels of the natriuretic peptides ANP and BNP in association with enhanced cyclic GMP levels, paralleled by higher cGMP-dependent protein kinase type I (cGKI) protein levels PMID: 26254181
  3. Angiotensin IV stimulates ANP secretion and concentration via the IRAP and PI3K-Akt-mTOR signaling pathway. PMID: 25451332
  4. augmentation of inhibitory effect on basal motility by ANP in experimental colitis may be due an increased expression of colonic natriuretic peptide receptor-A mRNA PMID: 26660905
  5. ANP mRNA and protein are decreased in diabetic cardiomyopathy. PMID: 26446774
  6. This is due to the fact that ANP is the main hormone of the natriuretic peptide system involved in the regulation of blood pressure in normal conditions, while BNP is the principal regulator of pressure in cardiovascular pathology PMID: 27228659
  7. These results suggest that chronic treatment with nimesulide or NS-398 attenuated hypertension and cardiac hypertrophy partly through ANP release in 2K1C rats. PMID: 25846103
  8. expression arrhythmic in heart and not influenced by rotating light-dark regimens PMID: 25669688
  9. These findings demonstrate an important contributory role of ANP in the OT induced protection in myocardial ischaemia reperfusion. OT also reduced lipid peroxidation with a NO-dependent mechanism. PMID: 25126708
  10. BMP antagonist Noggin was able to inhibit stretch and hypertrophic agonist induced BNP and ANP expression. PMID: 25218476
  11. These findings suggest that HIF-1alpha and ANP, synthesized by the kidney, are involved in an adaptive mechanism in response to a sodium overload. PMID: 24689065
  12. Resistance training did not induce any change in vasopressin levels, and atrial natriuretic peptide levels did not change significantly. PMID: 23551170
  13. NPPA acts on the NPR1 receptor, is expressed in rat dorsal root and trigeminal ganglia, and the spinal cord. PMID: 25123163
  14. Provide direct evidence for the presence and synthesis of ANP and its receptors in both neuronal and non-neuronal cells within the cochlear spiral ganglion. PMID: 24333928
  15. the present study delivers a cooperative mechanism where Anxa6 potentiates ANP-dependent counterhypertrophic responses in cardiomyocytes by facilitating regulated traffic of pro-ANP. PMID: 24403064
  16. We conclude that D1R may play a major role in the ANP resistance observed in puromycin aminonucleoside-induced nephrotic syndrome . PMID: 23956981
  17. a direct evidence for the presence and synthesis of ANP as well as its receptors in the cochlear Spiral ganglion neurons, suggesting a possible role for ANP in modulating the neuronal functions of Spiral ganglion neurons via its receptors PMID: 23578746
  18. Endogenously released ACh tonically stimulates ANP secretion from atrial cardiomyocytes via activation of M2 mAChR-Gi/o-KACh(+) channel signaling. PMID: 23913708
  19. These results outline a PARP-1-dependent signal transduction mechanism that links contraction rate and Ca(2+) mobilization with the expression of genes underlying morphological changes in cardiomyocytes. PMID: 21635224
  20. ANP might be synthesized and secreted by marginal cells of stria vascularis, and it could play an important role in modulating the microenvironment of the inner ear. In addition, ANP might contribute to development and growth process of cochlea. PMID: 21552198
  21. ANF signals via natriuretic peptide receptor-C coupled to the phospholipase C-protein kinase C pathway to increase secretin-induced efflux of cAMP, probably through MPR-4 in pancreas PMID: 21237168
  22. Comparison of vasopressin (VP) to atrial natriuretic peptide (ANP) suggests that in early resistance training, VP release to bloodstream is higher than ANP release; the antidiuretic action of VP should prevail over ANP. PMID: 21287975
  23. Syntaxin-4 and VAMP-1 and VAMP-2 regulate cardiac myocyte exocytosis of ANP. PMID: 20801128
  24. The right ventricle is not a major source of cardiac ANP release in normoxia- or hypoxia-adapted rats. PMID: 20691705
  25. Results describe the involvement of myogenin and GATA4, two tissue-specific transcription factors, and the atrial natriuretic factor promoter, in cardiac transcriptional activity. PMID: 20384792
  26. found a close correlation between pulmonary embolism degree and gene-expression of ANP, and BNP in the cardiac chambers with a selective increase in the right chambers of the heart PMID: 20559433
  27. Results suggest that urotensin-II stimulates high stimulation frequency-induced ANP secretion partly through the urotensin receptor and the PLC/PI3K/PKC pathway. PMID: 19896516
  28. Leptin inhibits ANP secretion through nitric oxide without changing basal or isoproterenol-induced ANP secretion in rats. PMID: 20071611
  29. Mechanical overload in combined models of hypertension determines the evolution of hypertrophy and synthesis and secretion of ANP and BNP. PMID: 20139323
  30. The distribution of ANP after local irradiation of the rat heart depends on the severity of the pathological/structural changes (i.e. myocyte degeneration and fibrosis). PMID: 12020441
  31. ANP reduced TNFalpha release possibly by influencing post-translational processing of TNFalpha in LPS-activated KCs. In addition, we demonstrated that ANP enhances phagocytosis in KCs. PMID: 12022755
  32. ANP expression increased in rat atria throughout the prenatal and postnatal periods, except for a decrease in ANP expression in ventricles from prenatal day 21 onwards; it may have antimitogenic/antiproliferative effects in trabecular myocytes PMID: 12389741
  33. role in hypoxic activation of atrial natriuretic peptide gene promoter PMID: 12413399
  34. antral ANP and somatostatin secretion are linked by paracrine feedback pathways: endogenous ANP, acting via the NPR-A receptor, stimulates somatostatin secretion, and endogenous somatostatin, acting via the sst2 receptor, inhibits ANP secretion PMID: 12527142
  35. Mechanisms of hepatocyte protection against hypoxic injury by atrial natriuretic peptide. ANP recruits 2 independent signal pathways, which further transduce ANP signals to p38 MAPK, that are responsible for ANP protection against hypoxic injury. PMID: 12540777
  36. findings support that ANF exerts a stimulatory effect on pancreatic exocrine secretion mediated by NPR-C receptors coupled to the phosphoinositide pathway PMID: 12829435
  37. Cardiac expression of ANF demonstrated a time-related increase in ANF mRNA. PMID: 14575315
  38. The present study is the first to show linkage between Nppa marker and blood pressure in the rat. p. 935 PMID: 14678232
  39. Atrial natriuretic peptide expressed in the supraoptic nucleus acts as a peptidergic neurotransmitter involved in water and salt regulation during pregnancy and postpartum. PMID: 15117337
  40. Recruitment of PAM-1 to secretory vesicles depends on intact N-terminal proANP and on the lumenal domain of PAM-1. Conversely, PAM-1 participates in shaping the proANP-secretory vesicles. PMID: 15539631
  41. ANP induces MKP-1. ANP is a novel endogenous activator of endothelial Rac1 and Nox/Nox2. PMID: 15569826
  42. The downregulation of ANP and NPRC in retinas of diabetic rats suggests a role for this peptide in experimental diabetic retinopathy. PMID: 15789000
  43. ANP interferes with lysophosphatydic acid-induced DNA synthesis and ROS production. PMID: 16010968
  44. SERCA2alpha gene down-regulation in the non-infarcted myocardium of rats with MI does not correlate with ANP gene upregulation, suggesting that the two genes are not antithetically regulated. PMID: 16087130
  45. Atrial natriuretic peptides are located in mast cell secretory granules and released by mechanism of degranulation. PMID: 16246270
  46. The ANP might play a role on the synthesis of the oxytocin since ANP and its mRNA appear earlier than oxytocin. PMID: 16377580
  47. atrial natriuretic peptide, urodilatin, guanylin and uroguanylin interact in rat kidney PMID: 16814407
  48. The ET-1-induced increase in cell area, ANF mRNA expression and (3)H-phenylalanine incorporation in ET-1-treated NRVM were decreased by NHE-1 or NCX(rev) inhibition. PMID: 16859700
  49. The results indicate that distinct differences in ANF transcriptional regulation exist in vivo in the adult heart as compared with neonatal cardiomyocytes. PMID: 16909307
  50. Blocking nuclear translocation and subsequent binding of pSmad2 and pSmad3 to TGF-beta-Smad response elements in ECM genes may be responsible for the inhibitory effects of ANP on TGF-beta-induced expression of ECM molecules. PMID: 17038494

Show More

Hide All

Subcellular Location Secreted
Protein Families Natriuretic peptide family
Database Links

KEGG: rno:24602

STRING: 10116.ENSRNOP00000011005

UniGene: Rn.2004

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1