Rat Beclin-1(BECN1) ELISA kit

Code CSB-EL002658RA
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details


This Rat BECN1 ELISA Kit was designed for the quantitative measurement of Rat BECN1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name beclin 1, autophagy related
Alternative Names Becn1Beclin-1 ELISA kit; Coiled-coil myosin-like BCL2-interacting protein ELISA kit; Protein GT197) [Cleaved into: Beclin-1-C 35 kDa; Beclin-1-C 37 kDa] ELISA kit
Abbreviation BECN1
Uniprot No. Q91XJ1
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat BECN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %86
Range %83-90
1:2Average %95
Range %91-99
1:4Average %91
Range %87-96
1:8Average %92
Range %87-95
The recovery of rat BECN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-98
EDTA plasma (n=4)9086-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
102.123 1.982 2.053 1.964
51.539 1.523 1.531 1.442
2.51.017 1.001 1.009 0.920
1.250.691 0.687 0.689 0.600
0.6250.422 0.412 0.417 0.328
0.3120.268 0.259 0.264 0.175
0.1560.166 0.161 0.164 0.075
00.091 0.087 0.089  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. May play a role in antiviral host defense.; Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
Gene References into Functions
  1. Results show decreased SRF levels in the substantia nigra (SN) of rotenone-treated rats that was associated with the loss of tyrosine hydroxylase (TH)-positive neurons. SRF expression was also reduced in rotenone-treated PC12 cells in vitro. In addition, Srf knockdown augmented rotenone-induced toxicity in PC12 cells. In contrast, overexpression of Srf attenuated the cells' sensitivity to rotenone. PMID: 29196028
  2. Expression of Beclin-1 and LAMP-2 in Rat Hippocampus under Conditions of Simulated Gravity Overload in the Caudocranial Vector PMID: 29797121
  3. Data suggested that Beclin-1-mediated autophagy impairs the expression and function of cleaved caspase-8 to protect against cadmium-induced activation of apoptosis through Fas/FasL signaling pathway. PMID: 28428545
  4. that after Caspase 3-mediated Beclin 1 cleavage at 146/149 sites, the N-terminal fragments of Beclin 1 may partially translocate into nuclei in neurons subjected to AD-like injury PMID: 28964771
  5. Lutein-induced autophagy was mediated by the upregulation of BECN1 in IEC-6 cells. PMID: 28893091
  6. treatment with an autophagy inhibitor decreased LC3II expression.However, miR-30b overexpression decreased Pi-induced expression of autophagy-related marker genes such as BECN1, ATG5, and LC3b, whereas miR-30b downregulation increased Pi-induced expression of these genes. In addition, we found that miR-30b PMID: 28578340
  7. These results suggest that EA protects against ischemic stroke through the inhibition of autophagosome formation and autophagy, which is mediated through the mTORC1-ULK complex-Beclin1 pathway PMID: 26647915
  8. in-vitro genetic depletion of beclin-1 in high glucose treated adult rat cardiomyocytes markedly inhibited the level of autophagy and subsequent apoptotic cell death. PMID: 26386349
  9. Hypoxia-affected fetal renal development was associated with renal apoptosis and Beclin 1 signaling-mediated autophagy. PMID: 24872334
  10. Beclin 1 has an essential role as a regulator between autophagy and apoptosis in the setting of neointimal formation PMID: 25238224
  11. veal a novel function of GA binding protein in the regulation of autophagy via transcriptional activation of the BECN1-PIK3C3 complex PMID: 25046113
  12. These results suggest that IPC inhibits Beclin 1-dependent excessive autophagy in reperfusion phase and cooperates with anti-apoptosis pathway to diminish the cell death induced by the myocardial I/R injury. PMID: 23392707
  13. Significantly higher level of Beclin-1 and LC3B-II/LC3B-I ratio (both definitive autophagy biomarkers) was observed in the fibrillated myocardium, versus tissue from the nonfibrillated hearts PMID: 23590156
  14. Rhes robustly binds the autophagy regulator Beclin-1, decreasing its inhibitory interaction with Bcl-2 independent of JNK-1 signaling. PMID: 24324270
  15. Level of beclin-1 in acute on chronic liver failure was lower than control. PMID: 24579466
  16. The results of yhis study found that levels of autophagy markers such as LC3 and Beclin 1 are elevated in the GABAergic interneurons of the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain. PMID: 23665054
  17. Beclin-1 upregulation results in enhanced levels of autophagy. PMID: 22917477
  18. beta-asarone can increase Bcl-2 level, finally interfere with the functions of Beclin 1 during the execution of autophagy. PMID: 22306244
  19. Inhibition of autophagy by Beclin 1 knockdown can attenuate the secondary thalamic damage after focal cerebral infarction. PMID: 22108007
  20. beclin1 may be involved in the pathogenesis of diabetic nephropathy with the mechanism related to the process of programmed cell death. PMID: 21866636
  21. Beclin 1-independent autophagy is an important contributor to both the caspase-dependent and -independent components of neuronal apoptosis and may be considered as an important therapeutic target in neural conditions involving apoptosis PMID: 21646862
  22. Data reveal that Beclin 1 and ATG5 play key roles in morphine-induced autophagy, which may contribute to morphine-induced neuronal injury. PMID: 20190558
  23. Hypoxia triggers a feedback mechanism that delays apoptosis of oxygen-deprived malignant intestinal epithelial cells and is driven by hypoxia-induced Beclin 1 downregulation. PMID: 19901551
  24. The inhibition of autophagy by urocortin is mediated in part by inhibition of Beclin1 expression, an effect which is mediated by activation of the PI3 kinase/Akt pathway but which does not involve activation of p42/p44 MAPK. PMID: 16697404
  25. Beclin 1 protein began to elevate at 1 h time point post-TBI in neurons, 3 d later in astrocytes, and peaked at about 8 d post-TBI. PMID: 18500386
  26. stimulation of autophagy by -methyl D-aspartate 2B glutamate signaling may be regulated by redistribution of Beclin-1 in membrane rafts PMID: 19335206
  27. the AMPK-MEK/ERK-TSC-mTOR pathway regulation of Beclin 1 represents different thresholds responsible for a protective or destructive autophagy PMID: 19520853
  28. Data show that RNA interference-mediated downregulation of Beclin1 improves outcomes after transient middle cerebral artery occlusion. PMID: 19574998

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Subcellular Location Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Cytoplasmic vesicle, autophagosome.; [Beclin-1-C 35 kDa]: Mitochondrion. Nucleus. Cytoplasm.; [Beclin-1-C 37 kDa]: Mitochondrion.
Protein Families Beclin family
Database Links

KEGG: rno:114558

STRING: 10116.ENSRNOP00000027868

UniGene: Rn.2776

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