Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

Instructions
Code CSB-E12776r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name C-telopeptide of type Ⅰ collagen,CTX-
Alternative Names C-telopeptide of type 1 collagen,CTX-1,C-telopeptide of type I collagen,CTX-I
Abbreviation CTX-
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-2000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 89-97  
1:2 Average % 92  
Range % 88-96  
1:4 Average % 98  
Range % 94-102  
1:8 Average % 84  
Range % 80-88  
Recovery
The recovery of rat CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-102  
EDTA plasma (n=4) 86 82-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.353 0.354 0.354    
750 0.468 0.475 0.472    
375 0.616 0.605 0.611    
125 1.011 0.995 1.003    
25 1.512 1.594 1.553    
0 2.013 2.114 2.064    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Q&A
Q:

I am writing to ask you a question about how to experiment.
I'd like to experiment with kit #CSB-E12776r.
Sample type is serum.
When diluting a sample, what solution would you recommend?

A:
Thanks for your inquiry. Regarding this item,#CSB-E12776r,there is no indeed recommadated dilution factors.Usually there is no need for dilutions and you can do pre-testing and research for the best dilution factor if you need dilutions.Series 0;1:10;1:100 is advised.(you can consider use PBS as diluent solution).
If any further questions,pls do not hesitate to tell me.
Q:

What's the antibody information of this kit?

A:
Thanks for your inquiry. Pls check our information: CSB-E12776r: Coated anti. Goat against rabbit secondary antibody; Antigen Specific anti: Rabbit polyclonal antibody; Pls let me know if you have any further questions. Thank you.
 Customer Reviews
  • Sample type: Serum
    Sample species: Rat
    Review:Serum concentrations of biomarkers, including TGF-β1, TNFα, IL-1β, IL-6, IL-18, CRP, and BNP were measured by Elisa kits following manufacture instructions. PMID: 29326134
  • Sample type: Plasma
    Sample species: Rat
    Review: Enzyme-linked immunosorbent assay (ELISA) kits were used to determine leptin and adiponectin, insulin and C-peptide , osteocalcin, ALP, CTX, and NTX, and estradiol levels in Molecules 2018, 23, 1205 11 of 14 plasma according to the manufacturer's protocol.
    PMID: 29772836
  • Sample type: Serum
    Sample species: Rat
    Review: Serum biomarkers of bone formation, N-terminal propeptide of type I procollagen (PINP) and of bone resorption, C-terminal telopeptide of type I collagen (CTX-I) indicated that structural bone changes observed following injury are mainly caused by the decreased bone resorption with mild change in bone formation. Values represent the mean ± standard deviation (SD). Each group consisted of 6 rats.*P< 0.05 compared to Sham by unpaired t-test; ^ P < 0.05 compared to Control; #P < 0.05 compared to CS; $< 0.05 compared to CS+IB.
    PMID: 29108027
  • Sample type: Serum
    Sample species: Rat
    Review: 1.Ctrl; 2.Sham(no ovariectomy); 3.Ovariectomy; 4.Ovariectomy+E2; 5.Ovariectomy+sample1; 6.Ovariectomy+sample2; 7.Ovariectomy+sample3; 8.Ovariectomy+sample4

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