| Description |
The Rat C-telopeptide-of-type I (CTX-I) ELISA kit is a ready-to-use microwell, strip plate ELISA kit for the quatitative measurement of rat CTX-I in biological samples, including serum, plasma, and tissue homogenates. It empolys the competitive ELISA technique in the detection of target analytes in the samples. This ELISA kit has been quality controlled in multiple characterisitics, including sensitivity, specificity, precision, linearity, and recovery. See the product instructions to obtain more information.
Standards or samples are added to the appropriate microtiter plate wells pre-coated with goat-anti-rabbit CTX-I antibody. HRP-conjugated CTX-I and anti-rat CTX-I antibody are pipped into the wells, where CTX-I in the sample or standards competes with the HRP labeled CTX-I for limited binding sites on the anti-rat CTX-I antibody. these Ag-Ab complexes are captured by the antibody immobilized on the plate. The solution color turns blue after the addition of substrate A and B. The color reaction is immediately terminated after the addition of stop solution. And the color changes from blue to yellow. The color develops oppositely to the amount of CTX-I in the sample, and the intensity of the color is measured by Microplate reader at 450 nm.
CTX-I is a sensitive and variable marker of bone resorption. Shereen M Olama etc, showed that serum CTX-I level was significantly elevated in patients with fibromyalgia syndrome (FMS) and that increased experssion of CTX-I was associated with the disease severity. Increased CTX-I may cause the early development of osteoporosis. |
| Target Name |
C-telopeptide of type Ⅰ collagen,CTX- |
| Alternative Names |
C-telopeptide of type 1 collagen,CTX-1,C-telopeptide of type I collagen,CTX-I |
| Abbreviation |
CTX- |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
25 pg/mL-2000 pg/mL |
| Sensitivity |
31.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others
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| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:2 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:4 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:8 |
Average % |
84 |
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| Range % |
80-88 |
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| Recovery |
| The recovery of rat CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
92-102 |
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| EDTA plasma (n=4) |
86 |
82-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
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| 2000 |
0.353 |
0.354 |
0.354 |
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| 750 |
0.468 |
0.475 |
0.472 |
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| 375 |
0.616 |
0.605 |
0.611 |
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| 125 |
1.011 |
0.995 |
1.003 |
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| 25 |
1.512 |
1.594 |
1.553 |
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| 0 |
2.013 |
2.114 |
2.064 |
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| Materials provided |
- A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-human CTX-I antibody.
- Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Anti-rat CTX-I Antibody 1 x 6 ml -- Act as the detection antibody.
- HRP-conjugated CTX-I 1 x 6 ml -- Bind to the CTX-I antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
- Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
- Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
- Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
- Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
