Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

Code CSB-E12776r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name C-telopeptide of type Ⅰ collagen,CTX-
Alternative Names C-telopeptide of type 1 collagen,CTX-1,C-telopeptide of type I collagen,CTX-I
Abbreviation CTX-
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-2000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 89-97  
1:2 Average % 92  
Range % 88-96  
1:4 Average % 98  
Range % 94-102  
1:8 Average % 84  
Range % 80-88  
The recovery of rat CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-102  
EDTA plasma (n=4) 86 82-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.353 0.354 0.354    
750 0.468 0.475 0.472    
375 0.616 0.605 0.611    
125 1.011 0.995 1.003    
25 1.512 1.594 1.553    
0 2.013 2.114 2.064    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Q&A and Customer Reviews


I am writing to ask you a question about how to experiment.
I'd like to experiment with kit #CSB-E12776r.
Sample type is serum.
When diluting a sample, what solution would you recommend?

Thanks for your inquiry. Regarding this item,#CSB-E12776r,there is no indeed recommadated dilution factors.Usually there is no need for dilutions and you can do pre-testing and research for the best dilution factor if you need dilutions.Series 0;1:10;1:100 is advised.(you can consider use PBS as diluent solution).
If any further questions,pls do not hesitate to tell me.

What's the antibody information of this kit?

Thanks for your inquiry. Pls check our information: CSB-E12776r: Coated anti. Goat against rabbit secondary antibody; Antigen Specific anti: Rabbit polyclonal antibody; Pls let me know if you have any further questions. Thank you.
 Customer Reviews
  • Sample type: Serum
    Sample species: Rat
    Review:Serum concentrations of biomarkers, including TGF-β1, TNFα, IL-1β, IL-6, IL-18, CRP, and BNP were measured by Elisa kits following manufacture instructions. PMID: 29326134
  • Sample type: Plasma
    Sample species: Rat
    Review: Enzyme-linked immunosorbent assay (ELISA) kits were used to determine leptin and adiponectin, insulin and C-peptide , osteocalcin, ALP, CTX, and NTX, and estradiol levels in Molecules 2018, 23, 1205 11 of 14 plasma according to the manufacturer's protocol.
    PMID: 29772836
  • Sample type: Serum
    Sample species: Rat
    Review: Serum biomarkers of bone formation, N-terminal propeptide of type I procollagen (PINP) and of bone resorption, C-terminal telopeptide of type I collagen (CTX-I) indicated that structural bone changes observed following injury are mainly caused by the decreased bone resorption with mild change in bone formation. Values represent the mean ± standard deviation (SD). Each group consisted of 6 rats.*P< 0.05 compared to Sham by unpaired t-test; ^ P < 0.05 compared to Control; #P < 0.05 compared to CS; $< 0.05 compared to CS+IB.
    PMID: 29108027
  • Sample type: Serum
    Sample species: Rat
    Review: 1.Ctrl; 2.Sham(no ovariectomy); 3.Ovariectomy; 4.Ovariectomy+E2; 5.Ovariectomy+sample1; 6.Ovariectomy+sample2; 7.Ovariectomy+sample3; 8.Ovariectomy+sample4

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