Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

Product Details

Citations

Q&A and Customer Reviews

Most popular with customers

Your Good Partner in Biology Research

Tel : 301-363-4651

Home
Kit
ELISA Kit
Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

Name: Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit
Brand: CUSABIO
SKU: CSB-E12776r

^Instructions

Code	CSB-E12776r
Size	96T,5×96T,10×96T
See More Details
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Citations
Related Products
Q&A and Customer Reviews

Target Name

C-telopeptide of type Ⅰ collagen,CTX-

Alternative Names

C-telopeptide of type 1 collagen,CTX-1,C-telopeptide of type I collagen,CTX-I

Abbreviation

CTX-

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

25 pg/mL-2000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	92
	Range %	88-96
1:4	Average %	98
	Range %	94-102
1:8	Average %	84
	Range %	80-88

Recovery

The recovery of rat CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-102
EDTA plasma (n=4)	86	82-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.353	0.354	0.354
750	0.468	0.475	0.472
375	0.616	0.605	0.611
125	1.011	0.995	1.003
25	1.512	1.594	1.553
0	2.013	2.114	2.064

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Immune modulatory and antioxidant effects of locally administrated vitamin C in experimental periodontitis in rats Z Aytekin,Acta Odontol Scand,2020
Effects of C-peptide replacement therapy on bone microarchitecture parameters in streptozotocin-diabetic rats. S Maurotti,bioRxiv,2020
Vaspin antagonizes high fat-induced bone loss in rats and promotes osteoblastic differentiation in primary rat osteoblasts through Smad-Runx2 signaling Wang H,Nutrition & Metabolism,2020
Flaxseed oil ameliorated high-fat-diet-induced bone loss in rats by promoting osteoblastic function in rat primary osteoblasts Chen F, et al,Nutrition & Metabolism,2019
Histological and Biochemical Investigation of the Effects of Low Intensity Pulsed Ultrasoundon Orthodontic Tooth Movement Mine Gecgelen Cesur, et al,Konuralp Medical Journal,2019
Effects of curcumin and melatonin on bone formation in orthopedically expanded suture in rats: A biochemical, histological and immunohistochemical study M. G. Cesur.et al,ORTHOD CRANIOFAC RES,2018
Soybean-Hop Alleviates Estrogen Deficiency-Related Bone Loss and Metabolic Dysfunction in Ovariectomized Rats Fed a High-Fat Diet Dasom Noh.et al,Molecules,2018
Animal model having hypoparathyroidism and method for producing the same Han Su.et al,/,2018
Evaluation of estrogenic potential by herbal formula, HPC 03 for in vitro and in vivo Chang BY.et al,Reproduction,2018
Advanced oxidation protein products accelerated the bone loss and weakened bone strength in ovariectomised rats Shuai Zheng.et al,Int J Clin Exp Pathol ,2017
Promotion effect of extracts from plastrum testudinis on alendronate against glucocorticoid-induced osteoporosis in rat spine Hui Ren.et al,Sci Rep,2017
Local application of an ibandronate/collagen sponge improves femoral fracture healing in ovariectomized rats Guo J.et al,PLoS One,2017
Combination breast cancer chemotherapy with doxorubicin and cyclophosphamide damages bone and boneMarrow in a female rat model Fan C.et al,Breast Cancer Res Treat,2017
Extracts from plastrum testudinis reverse glucocorticoid-induced spinal osteoporosis of rats via targeting osteoblastic and osteoclasticMarkers De Liang.et al,Biomedicine & Pharmacotherapy,2016
Effect of QiShenYiQi Pill on Myocardial Collagen Metabolism in Rats with Partial Abdominal Aortic Coarctation Lv S.et al,Evid Based Complement Alternat Med.,2015
Adipose tissue-derived stem cells in the ovariectomy-induced postmenopausal osteoporosis rat model Jeong J H.et al,Tissue Engineering and Regenerative Medicine,2015
Wnt3a-producing fibroblasts in ovariectomy-induced osteoporosis in a rat model Je Hoon Jeong. et al,Tissue Engineering and Regenerative Medicine,2015
Combination chemotherapy with cyclophosphamide, epirubicin and 5-fluorouracil causes trabecular bone loss, boneMarrow cell depletion andMarrow adiposity in female rats Fan C. et al,J Bone Miner Metab,2015
Ameliorative potential of stem bromelain on lead-induced toxicity in Wistar rats Al-Otaibi WR. et al,Acta Biol Hung,2015
Effect of whole body vibration therapy on circulating serotonin levels in an ovariectomized rat model of osteoporosis Wei QS et al,Iran J Basic Med Sci,2014
Effects of Chronic Stress and Alendronate Therapy on the Osseointegration of Titanium Implants Conte Neto N et al,Clin Implant Dent Relat Res.,2013

■ Q&A

I am writing to ask you a question about how to experiment.
I'd like to experiment with kit #CSB-E12776r.
Sample type is serum.
When diluting a sample, what solution would you recommend?

Thanks for your inquiry. Regarding this item,#CSB-E12776r,there is no indeed recommadated dilution factors.Usually there is no need for dilutions and you can do pre-testing and research for the best dilution factor if you need dilutions.Series 0；1:10；1:100 is advised.（you can consider use PBS as diluent solution）.
If any further questions,pls do not hesitate to tell me.

What's the antibody information of this kit?

Thanks for your inquiry. Pls check our information: CSB-E12776r: Coated anti. Goat against rabbit secondary antibody; Antigen Specific anti: Rabbit polyclonal antibody; Pls let me know if you have any further questions. Thank you.

■ Customer Reviews

Sample type: Serum
Sample species: Rat
Review:Serum concentrations of biomarkers, including TGF-β1, TNFα, IL-1β, IL-6, IL-18, CRP, and BNP were measured by Elisa kits following manufacture instructions. PMID: 29326134
Sample type: Plasma
Sample species: Rat
Review: Enzyme-linked immunosorbent assay (ELISA) kits were used to determine leptin and adiponectin, insulin and C-peptide , osteocalcin, ALP, CTX, and NTX, and estradiol levels in Molecules 2018, 23, 1205 11 of 14 plasma according to the manufacturer's protocol.
PMID: 29772836
Sample type: Serum
Sample species: Rat
Review: Serum biomarkers of bone formation, N-terminal propeptide of type I procollagen (PINP) and of bone resorption, C-terminal telopeptide of type I collagen (CTX-I) indicated that structural bone changes observed following injury are mainly caused by the decreased bone resorption with mild change in bone formation. Values represent the mean ± standard deviation (SD). Each group consisted of 6 rats.*P< 0.05 compared to Sham by unpaired t-test; ^ P < 0.05 compared to Control; #P < 0.05 compared to CS; $< 0.05 compared to CS+IB.
PMID: 29108027
Sample type: Serum
Sample species: Rat
Review: 1.Ctrl; 2.Sham(no ovariectomy); 3.Ovariectomy; 4.Ovariectomy+E2; 5.Ovariectomy+sample1; 6.Ovariectomy+sample2; 7.Ovariectomy+sample3; 8.Ovariectomy+sample4

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mouse 1,25-dihydroxyvitamin D3 (DVD/DHVD3) ELISA kit

Detect Range: 1000 fmol/L-5000 fmol/L

Sensitivity: 250 fmol/L
Human α2 macroglobulin,α2 MG ELISA Kit

Detect Range: 1.25 μg/mL-20 μg/mL

Sensitivity: 1.25 μg/mL
Human Arachidonic Acid,AA ELISA Kit

Detect Range: 78.125 ng/mL-5000 ng/mL

Sensitivity: 69.748 ng/mL
Mouse Angiotensin converting enzyme,ACE ELISA Kit

Detect Range: 1.56 ng/mL-100 ng/mL

Sensitivity: 0.39 ng/mL
Rat adiponectin,ADP ELISA Kit

Detect Range: 0.156 ng/mL-10 ng/mL

Sensitivity: 0.039 ng/mL
Human Alpha-fetoprotein,AFP ELISA Kit

Detect Range: 1.563 ng/mL-100 ng/mL

Sensitivity: 1.332 ng/mL
Human microalbunminuria(MAU/ALB) ELISA kit

Detect Range: 0.078 μg/mL-5 μg/mL

Sensitivity: 0.019 μg/mL
Pig Albumin (Alb) ELISA Kit

Detect Range: 0.03 μg/mL-7000 μg/mL

Sensitivity: 0.03 μg/mL