Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

Instructions
Code CSB-E12776r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Rat C-telopeptide-of-type I (CTX-I) ELISA kit is a ready-to-use microwell, strip plate ELISA kit for the quatitative measurement of rat CTX-I in biological samples, including serum, plasma, and tissue homogenates. It empolys the competitive ELISA technique in the detection of target analytes in the samples. This ELISA kit has been quality controlled in multiple characterisitics, including sensitivity, specificity, precision, linearity, and recovery. See the product instructions to obtain more information.

Standards or samples are added to the appropriate microtiter plate wells pre-coated with goat-anti-rabbit CTX-I antibody. HRP-conjugated CTX-I and anti-rat CTX-I antibody are pipped into the wells, where CTX-I in the sample or standards competes with the HRP labeled CTX-I for limited binding sites on the anti-rat CTX-I antibody. these Ag-Ab complexes are captured by the antibody immobilized on the plate. The solution color turns blue after the addition of substrate A and B. The color reaction is immediately terminated after the addition of stop solution. And the color changes from blue to yellow. The color develops oppositely to the amount of CTX-I in the sample, and the intensity of the color is measured by Microplate reader at 450 nm.

CTX-I is a sensitive and variable marker of bone resorption. Shereen M Olama etc, showed that serum CTX-I level was significantly elevated in patients with fibromyalgia syndrome (FMS) and that increased experssion of CTX-I was associated with the disease severity. Increased CTX-I may cause the early development of osteoporosis.

Target Name C-telopeptide of type Ⅰ collagen,CTX-
Alternative Names C-telopeptide of type 1 collagen,CTX-1,C-telopeptide of type I collagen,CTX-I
Abbreviation CTX-
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-2000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 89-97  
1:2 Average % 92  
Range % 88-96  
1:4 Average % 98  
Range % 94-102  
1:8 Average % 84  
Range % 80-88  
Recovery
The recovery of rat CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-102  
EDTA plasma (n=4) 86 82-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.353 0.354 0.354    
750 0.468 0.475 0.472    
375 0.616 0.605 0.611    
125 1.011 0.995 1.003    
25 1.512 1.594 1.553    
0 2.013 2.114 2.064    
Materials provided
  • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-human CTX-I antibody.
  • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • Anti-rat CTX-I Antibody 1 x 6 ml -- Act as the detection antibody.
  • HRP-conjugated CTX-I 1 x 6 ml -- Bind to the CTX-I antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
  • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
  • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
  • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
  • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
4.5 - 2 reviews

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Sample type: Serum

Sample species: Rat

Review: Serum biomarkers of bone formation, N-terminal propeptide of type I procollagen (PINP) and of bone resorption, C-terminal telopeptide of type I collagen (CTX-I) indicated that structural bone changes observed following injury are mainly caused by the decreased bone resorption with a mild change in bone formation. Values represent the mean ± standard deviation (SD). Each group consisted of 6 rats.*P< 0.05 compared to Sham by unpaired t-test; ^ P < 0.05 compared to Control; #P < 0.05 compared to CS; $< 0.05 compared to CS+IB.

By Anonymous

Sample type: Serum

Sample species: Rat

Review: 1.Ctrl; 2.Sham(no ovariectomy); 3.Ovariectomy; 4.Ovariectomy+E2; 5.Ovariectomy+sample1; 6.Ovariectomy+sample2; 7.Ovariectomy+sample3; 8.Ovariectomy+sample4

By Anonymous

 Q&A
Q:

I am writing to ask you a question about how to experiment.
I'd like to experiment with kit #CSB-E12776r.
Sample type is serum.
When diluting a sample, what solution would you recommend?

A:
Thanks for your inquiry. Regarding this item,#CSB-E12776r,there is no indeed recommadated dilution factors.Usually there is no need for dilutions and you can do pre-testing and research for the best dilution factor if you need dilutions.Series 0;1:10;1:100 is advised.(you can consider use PBS as diluent solution).
If any further questions,pls do not hesitate to tell me.
Q:

What's the antibody information of this kit?

A:
Thanks for your inquiry. Pls check our information: CSB-E12776r: Coated anti. Goat against rabbit secondary antibody; Antigen Specific anti: Rabbit polyclonal antibody; Pls let me know if you have any further questions. Thank you.

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