Rat Caspase-9,Casp-9 ELISA Kit

Instructions
Code CSB-E08863r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Caspase-9,Casp-9
Abbreviation Casp-9
Uniprot No. Q9JHK1
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %90-99
1:2Average %95
Range %91-102
1:4Average %98
Range %94-103
1:8Average %102
Range %96-106
Recovery
The recovery of rat Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)10096-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40001.846 1.946 1.896 1.752
20001.442 1.542 1.492 1.348
10000.986 1.016 1.001 0.857
5000.546 0.556 0.551 0.407
2500.320 0.330 0.325 0.181
1250.266 0.278 0.272 0.128
62.50.204 0.207 0.206 0.062
00.143 0.144 0.144  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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