Rat Complement fragment 5,C5 ELISA Kit

Code CSB-E14363r
Size 96T,5×96T,10×96T
Price Request a Quote
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Product Details

Target Name
complement component 5
Alternative Names
C5Complement C5 [Cleaved into: C5a anaphylatoxin] ELISA kit; Fragment ELISA kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
9.38 ng/mL-600 ng/mL
Sensitivity
2.34 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat C5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:200

Average %

104

Range %

97-111

1:400

Average %

101

Range %

94-108

1:800

Average %

98

Range %

93-103

1:1600

Average %

91

Range %

84-97

Recovery

The recovery of rat C5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

104

99-110

EDTA plasma (n=4)

95

88-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

600

2.218

2.134

2.176

2.070

300

1.864

1.767

1.815

1.710

150

1.346

1.298

1.322

1.216

75

0.937

0.929

0.933

0.827

37.5

0.601

0.611

0.606

0.500

18.75

0.436

0.453

0.445

0.339

9.38

0.302

0.294

0.298

0.192

0

0.106

0.105

0.106

 

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.; Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Subcellular Location
Secreted.
Database Links
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