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Rat Cystatin C,Cys-C ELISA Kit

Rat Cystatin C,Cys-C ELISA Kit

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Citations (22) Protocol MSDS

Code	CSB-E08385r
Size	96T,5×96T,10×96T
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Product Details
Citations (22)
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Target Name

cystatin C

Alternative Names

Cst3 ELISA Kit; Cystatin-C ELISA Kit; Cystatin-3 ELISA Kit

Abbreviation

CST3

Uniprot No.

P14841

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, homogenates.

Detection Range

0.312 ng/ml-20 ng/ml

Sensitivity

0.078 ng/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	2.490	2.550	2.588	2.467	2.527	2.555
SD	0.031	0.039	0.046	0.041	0.048	0.056
CV(%)	3.407	4.225	4.941	4.530	5.229	6.061

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:2

Average %

Range %

93-99

1:4

Average %

Range %

90-96

1:8

Average %

Range %

91-104

1:16

Average %

Range %

89-95

Recovery

The recovery of rat Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
20	2.564	2.587	2.576	2.404
10	1.923	1.934	1.929	1.757
5	1.329	1.368	1.349	1.177
2.5	0.912	0.937	0.925	0.753
1.25	0.582	0.598	0.590	0.418
0.625	0.412	0.436	0.424	0.252
0.312	0.311	0.317	0.349	0.177
0	0.170	0.174	0.172

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat CST3 ELISA Kit was designed for the quantitative measurement of Rat CST3 protein in serum, plasma, homogenates.. It is a Sandwich ELISA kit, its detection range is 0.312 ng/ml-20 ng/ml and the sensitivity is 0.078 ng/ml.

Citations

Abatement by Alhagi maurorum of lead-induced nephrotoxicity in rats: emphasis on Nrf2/HO-1 MM Algandaby,Future Journal of Pharmaceutical Sciences,2025
Sanqi oral solution ameliorates renal fibrosis by suppressing fibroblast activation via HIF-1α/PKM2/glycolysis pathway in chronic kidney disease D Hu, L Wang, Y Zhang, X Liu, Z Lu, H Li,Journal of Ethnopharmacology,2024
Linagliptin mitigates cisplatin-induced kidney impairment via mitophagy regulation in rats, with emphasis on SIRT-3/PGC-1α, PINK-1 and Parkin-2 MGED Ewees, G Mostafa-Hadeab, S Saber,Toxicology and Applied Pharmacology,2024
Clopidogrel protects against gentamicin-induced nephrotoxicity through targeting oxidative stress, apoptosis, and coagulation pathways AA Akila, RA Gad, MGED Ewees,Naunyn-Schmiedeberg's Archives of Pharmacology,2024
Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats MGED Ewees,Environmental toxicology and pharmacology,2023
Rosuvastatin and diosmetin inhibited the HSP70/TLR4 /NF-κB p65/NLRP3 signaling pathways and switched macrophage to M2 phenotype in a rat model of acute kidney injury induced by cisplatin F Feng,Biomedicine & pharmacotherapy,2024
Anandamide modulates WNT-5A/BCL-2, IP3/NFATc1, and HMGB1/NF-κB trajectories to protect against mercuric chloride-induced acute kidney injury DM Abdallah,Scientific reports,2023
Effect of perinatal exposure to glyphosate and its mixture with 2, 4-D and dicamba on rat dam kidney and thyroid function and offspring's health AO Docea,Environmental research,2023
2-Methoxyestradiol TPGS Micelles Attenuate Cyclosporine A-Induced Nephrotoxicity in Rats through Inhibition of TGF-β1 and p-ERK1/2 Axis MW Al-Rabia,Antioxidants,2022
Omeprazole Prevents Colistin-Induced Nephrotoxicity in Rats: Emphasis on Oxidative Stress, Inflammation, Apoptosis and Colistin Accumulation in Kidneys MZ Nasrullah,Pharmaceuticals,2022
Renoprotective Effect of Vardenafil and Avanafil in Contrast-Induced Nephropathy: Emerging Evidence from an Animal Model IE Zisis,Journal of Personalized Medicine,2022
Exogenous H2S Ameliorates High Salt-Induced Hypertension by Alleviating Oxidative Stress and Inflammation in the Paraventricular Nucleus in Dahl S Rats Yingying Liao,Cardiovascular Toxicology,2022
Involvement of NOX-4/JAK/STAT pathway in the protective effect of aprepitant against diclofenac-induced renal toxicity HM Hafez,Life Sciences,2022
Renoprotective effects of tropisetron through regulation of the TGF-β1, p53 and matrix metalloproteinases in streptozotocin-induced diabetic rats B Pourheydar,Chemico-Biological Interactions,2021
The renoprotective effect of glycyrrhizic acid in insulin-resistant rats exposed to aluminum involves the inhibition of TLR4/NF-κB signaling pathway NA Emara,Naunyn-Schmiedeberg's Archives of Pharmacology,2020
Protective Effects of Combination of Radix Astragali and Radix Salviae Miltiorrhizae on Kidney of Spontaneously Hypertensive Rats and Renal Intrinsic Cells Li W, et al,Chinese Journal of Integrative Medicine,2019
Tempol, a superoxide dismutase mimetic agent, reduces cisplatin-induced nephrotoxicity in rats Mohamed G. Ewees.et al,drug and chemical toxicology,2018
Ameliorative effect of ursolic acid on renal fibrosis in adenine-induced chronic kidney disease in rats RichaThakur.et al,Biomedicine & Pharmacotherapy,2018
Stimulation of ACE2/ANG/Mas Axis by Diminazene Ameliorates Alzheimer's Disease in the D-Galactose-Ovariectomized Rat Model: Role of PI3K/Akt Kamel AS.et al,Mol Neurobiol,2018
Nandrolone administration with or without strenuous exercise promotes overexpression of nephrin and podocin genes and induces structural and functional Tofighi A.et al,Toxicol Lett,2017
Relevance of cystatin-C, N-acetylglucosaminidase, and Interleukin-18 with the diagnosis of acute kidney injury induced by cadmium in rats Mohamed Ali OS.et al,J Biochem Mol Toxicol.,2017
Transplantation of insulin-producing cells to treat diabetic rats after 90% pancreatectomy Ya-Bin Yu. et al,World J Gastroenterol.,2015

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.

Gene References into Functions

Tanshinone IIA protects against cardiac hypertrophy in spontaneously hypertensive rats by inhibiting the Cys-C/Wnt signaling pathway. PMID: 28053285
These results indicate that Cd increases the urinary excretion of cystatin C, and they suggest that this effect may involve disruption of megalin-mediated uptake of cystatin C by epithelial cells of the proximal tubule. PMID: 26715107
High cystatin C is associated with proteinuria. PMID: 21551085
Triiodothyronine-induced increase in the production of cystatin C may be related to an increased cell metabolism and proteolysis control demand. PMID: 22360852
CysC plays a protective role under conditions of neuronal challenge by inducing autophagy via mTOR inhibition PMID: 20352108
Oxidants induce elevated cystatin C production from cardiac myocytes. Cystatin C plays a role in cardiac extracellular matrix remodelling. PMID: 20489058
We found several novel glycoforms of cystatin c in rat hippocampal stem cells, suggesting that N- and O-linked glycans with sialic acid are attached to cystatin C. PMID: 15197734
These results demonstrate that cystatin C has protective effects against various oxidative stresses that induce cell death. PMID: 16140167
endometrial expression of the cysteine protease inhibitor, Cst3, is modulated by estrogen during the peri-implantation period PMID: 16935259
These studies suggest alternate pathways and potential therapeutic targets of cell death associated with oxidative stress, CC, and lysosomal dysregulation. PMID: 17241700
Finally, in vivo studies using kidney-specific megalin knockout mice provide evidence that megalin mediates proximal tubular uptake of cystatin C. We conclude that megalin is an endocytic receptor of cystatin C in PTC. PMID: 17462596

Hide All

Subcellular Location

Secreted.

Protein Families

Cystatin family

Database Links

KEGG: rno:25307

STRING: 10116.ENSRNOP00000007175

UniGene: Rn.106351

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Rat Cystatin C,Cys-C ELISA Kit

Product Details

Citations

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Target Background