Target Name
cytochrome P450, family 19, subfamily A, polypeptide 1
Alternative Names
Cyp19a1 ELISA Kit; Arom ELISA Kit; Cyp19Aromatase ELISA Kit; EC 1.14.14.14 ELISA Kit; CYPXIX ELISA Kit; Cytochrome P-450AROM ELISA Kit; Cytochrome P450 19A1 ELISA Kit; Estrogen synthase ELISA Kit
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
18.75 pg/mL-1200 pg/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP19A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 101 | |
| Range % | 95-107 | |
| 1:2 | Average % | 91 | |
| Range % | 86-97 | |
| 1:4 | Average % | 88 | |
| Range % | 84-92 | |
| 1:8 | Average % | 95 | |
| Range % | 90-100 | |
Recovery
| The recovery of rat CYP19A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 85 | 80-90 | |
| EDTA plasma (n=4) | 96 | 91-103 | |
| | | | | | | |
| | | | | | | |
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 1200 | 2.452 | 2.272 | 2.362 | 2.237 | | | 600 | 1.705 | 1.832 | 1.769 | 1.644 | | | 300 | 1.286 | 1.316 | 1.301 | 1.176 | | | 150 | 0.829 | 0.880 | 0.855 | 0.730 | | | 75 | 0.504 | 0.469 | 0.487 | 0.362 | | | 37.5 | 0.301 | 0.285 | 0.293 | 0.168 | | | 18.75 | 0.203 | 0.211 | 0.207 | 0.082 | | | 0 | 0.127 | 0.122 | 0.125 | | |
|
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description
This Rat CYP19A1 ELISA Kit was designed for the quantitative measurement of Rat CYP19A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.
