Rat Cytochrome P450 19A1(CYP19A1) ELISA kit

Instructions
Code CSB-EL006394RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cytochrome P450, family 19, subfamily A, polypeptide 1
Alternative Names Cyp19a1 ELISA Kit; Arom ELISA Kit; Cyp19Aromatase ELISA Kit; EC 1.14.14.14 ELISA Kit; CYPXIX ELISA Kit; Cytochrome P-450AROM ELISA Kit; Cytochrome P450 19A1 ELISA Kit; Estrogen synthase ELISA Kit
Abbreviation CYP19A1
Uniprot No. P22443
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.68 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP19A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %101
Range %95-107
1:2Average %91
Range %86-97
1:4Average %88
Range %84-92
1:8Average %95
Range %90-100
Recovery
The recovery of rat CYP19A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8580-90
EDTA plasma (n=4)9691-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
12002.452 2.272 2.362 2.237
6001.705 1.832 1.769 1.644
3001.286 1.316 1.301 1.176
1500.829 0.880 0.855 0.730
750.504 0.469 0.487 0.362
37.50.301 0.285 0.293 0.168
18.750.203 0.211 0.207 0.082
00.127 0.122 0.125
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Subcellular Location Membrane, Peripheral membrane protein
Protein Families Cytochrome P450 family
Database Links

STRING: 10116.ENSRNOP00000000212

UniGene: Rn.21402

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