Rat Dehydroepiandrosterone,DHEA ELISA Kit

Code CSB-E08227r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Dehydroepiandrosterone,DHEA
Abbreviation
DHEA
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.05 ng/mL-10 ng/mL
Sensitivity
0.025 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %102
Range %98-107
1:2Average %92
Range %88-96
1:4Average %89
Range %85-93
1:8Average %100
Range %97-104
Recovery
The recovery of rat DHEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-92
EDTA plasma (n=4)9488-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1OD2Average
100.162 0.158 0.160
2.50.307 0.298 0.303
0.80.487 0.504 0.496
0.20.801 0.810 0.806
0.051.224 1.305 1.265
02.000 1.899 1.950
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat DHEA ELISA Kit was designed for the quantitative measurement of Rat DHEA protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.05 ng/mL-10 ng/mL and the sensitivity is 0.025 ng/mL .

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 Q&A
Q:

Please provide the following information regarding Rat Dehydroepiandrosterone, DHEA ELISA Kit (CSB-E08227r): What is the active ingredient of the stop solution and its concentration?
What is the type, concentration, and state (liquid/lyophilized) of the preservatives used?
What is the expression host of the standard?
which components have H202 and the concentration of H202 in them?Just to confirm this I am inquiring for 24 and 96 well of this kit. Please confirm if the information will be the same for both the 24T and 96T in regards to Rat Dehydroepiandrosterone, DHEA ELISA Kit (CSB-E08227r)?
**if the preservative/s are not liquid, what is the concentration of the component prior to reconstitution (while still lyophilized)?

A:
Thanks for your inquiry.
CSB-E08227r
Standard is purified DHEA.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000 dilution).
Sorry we can not disclose which components have H202 and the concentration of H202 in them.
For 24T and 96T, the component information is the same. Only the volume of reagents is different.
Pls let me know if you have any further questions. Thank you.
Q:

Can you please clarify if the preservative is liquid or lyophilized?
If it's lyophilized, please let us know concentration too.

A:
Thanks for your further inquiry. It is liquid.
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