Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

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Target Name

Granulocyte Colony Stimulating Factor,G-CSF

Alternative Names

Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit

Abbreviation

G-CSF

Uniprot No.

P48750

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-94
1:2	Average %	101
	Range %	97-104
1:4	Average %	95
	Range %	90-97
1:8	Average %	100
	Range %	95-103

Recovery

The recovery of rat G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	94	92-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.133	2.118	2.126	2.007
1000	1.837	1.776	1.807	1.688
500	1.294	1.282	1.288	1.169
250	0.889	0.878	0.884	0.765
125	0.588	0.607	0.598	0.479
62.5	0.361	0.349	0.355	0.236
31.25	0.222	0.216	0.219	0.100
0	0.118	0.120	0.119

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Data

Function	Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Gene References into Functions	Transplant vasculopathy depends on the simultaneous and complementary additive effects of granulocyte-macrophage colony-stimulating factor and cytokines, which have a continuous "cross talk." PMID: 28550897 Mesenteric mesothelial cells express GM-CSF receptor on the plasma membrane; during inflammation they synthesize and secrete GM-CSF that can stimulate GM-CSF receptor expression and mesothelial cell to macrophage-like cell transformation; in this transformation, GM-CSF (together with its receptor and other cytokines) has a major autocrine regulatory role; receptor-ligand internalization is essential for GM-CSF signalling PMID: 27364613 The findings that ephrinA1-PE38/GM-CSF had a high efficacy in the activation of the dendritic cells would facilitate the development of in vivo dendritic-cell vaccines for the treatment of gliomas in rats PMID: 25677907 Monocyte chemotactic protein-1 and GM-CSF may play an important role in the progression of experimental autoimmune myocarditis PMID: 12071442 GM-CSF exerts a neuromodulatory effect and may provide a new link of neuroimmune communication. PMID: 14557677 We identified the exclusive expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Paneth cells using single-cell reverse transcription-polymerase chain reaction and cDNA array. PMID: 14651956 GM-CSF may play a significant role in follicular development PMID: 15958400 GM-CSF signaling in the central nervous system can regulate energy homeostasis. PMID: 16276414 GM-CSF signaling as reflected by PU.1 expression and nuclear binding was restored with recombinant GM-CSF treatment in vitro. PMID: 16877635 GM-CSF acts as a neuroprotective protein in the CNS PMID: 17457367 Maternal administration of GM-CSF induced fetal lung maturation and this effect may be mediated, at least partly, by an increase in the eNOS expression. PMID: 17478029 GM-CSF stimulates neuronal differentiation of adult neural stem cells. PMID: 17953750 G-CSF improves and GM-CSF exacerbates early postinfarction LV remodeling in association with modulation of DC infiltration. PMID: 18832728 IL-1beta promotes tissue catabolism and destruction, whereas GM-CSF enhances tissue reconstruction PMID: 19022682 Show More Hide All
Subcellular Location	Secreted
Protein Families	GM-CSF family
Database Links	KEGG: rno:116630 STRING: 10116.ENSRNOP00000034235 UniGene: Rn.44285

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Check Code：

Code	CSB-E07340r
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.