Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

Code CSB-E07340r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P48750
Lead Time 3-5 working days
Abbreviation G-CSF
Target Name Granulocyte Colony Stimulating Factor,G-CSF
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %91
Range %86-94
1:2Average %101
Range %97-104
1:4Average %95
Range %90-97
1:8Average %100
Range %95-103
Recovery
The recovery of rat G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-96
EDTA plasma (n=4)9492-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.133 2.118 2.126 2.007
10001.837 1.776 1.807 1.688
5001.294 1.282 1.288 1.169
2500.889 0.878 0.884 0.765
1250.588 0.607 0.598 0.479
62.50.361 0.349 0.355 0.236
31.250.222 0.216 0.219 0.100
00.118 0.120 0.119  
References
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Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Subcellular Location Secreted
Protein Families GM-CSF family
Database Links

KEGG: rno:116630

STRING: 10116.ENSRNOP00000034235

UniGene: Rn.44285

Pathway Jak-STAT signaling pathway
TNF signaling pathway
Fc epsilon RI signaling pathway
Hematopoietic cell lineage
IL-17 signaling pathway
Natural killer cell mediated cytotoxicity
T cell receptor signaling pathway

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