Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

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Csf2 Proteins

Csf2 ELISA Kit

Csf2 Proteins

Csf2 ELISA Kit

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Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

Specification
Target

Code

CSB-E07340r

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P48750

Lead Time

3-5 working days

Abbreviation

G-CSF

Target Name

Granulocyte Colony Stimulating Factor,G-CSF

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-94
1:2	Average %	101
	Range %	97-104
1:4	Average %	95
	Range %	90-97
1:8	Average %	100
	Range %	95-103

Recovery

The recovery of rat G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	94	92-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.133	2.118	2.126	2.007
1000	1.837	1.776	1.807	1.688
500	1.294	1.282	1.288	1.169
250	0.889	0.878	0.884	0.765
125	0.588	0.607	0.598	0.479
62.5	0.361	0.349	0.355	0.236
31.25	0.222	0.216	0.219	0.100
0	0.118	0.120	0.119

References

BoneMarrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms Golle L.et al,Sci Rep,2017

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Function	Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Subcellular Location	Secreted
Protein Families	GM-CSF family
Database Links	KEGG: rno:116630 STRING: 10116.ENSRNOP00000034235 UniGene: Rn.44285
Pathway	Jak-STAT signaling pathway TNF signaling pathway Fc epsilon RI signaling pathway Hematopoietic cell lineage IL-17 signaling pathway Natural killer cell mediated cytotoxicity T cell receptor signaling pathway

Csf2 Protein

Csf2 ELISA Kit

Csf2 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP006045RA CSB-EP006045RA CSB-BP006045RA CSB-MP006045RA	Recombinant Rat Granulocyte-macrophage colony-stimulating factor(Csf2)	Yeast E.coli Baculovirus Mammalian cell

Csf2 ELISA Kit for Rattus norvegicus (Rat)

Code	Product Name	Sample Types	Sensitivity
CSB-E04570r	Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit	serum, plasma, tissue homogenates	1.56 pg/mL

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.