Rat IL-1 Receptor Like 1(IL1RL1)ELISA Kit

Code CSB-E14896r
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 1 receptor-like 1
Alternative Names
Il1rl1 ELISA Kit; Fit1 ELISA Kit; Interleukin-1 receptor-like 1 ELISA Kit; EC 3.2.2.6 ELISA Kit; Fit-1 ELISA Kit; Fos-responsive gene 1 protein ELISA Kit
Abbreviation
IL1RL1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL1RL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 80-92  
1:2 Average % 97  
Range % 91-104  
1:4 Average % 96  
Range % 91-101  
1:8 Average % 92  
Range % 86-96  
Recovery
The recovery of rat IL1RL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 90-100  
EDTA plasma (n=4) 90 85-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.317 2.252 2.285 2.153  
500 1.969 1.923 1.946 1.814  
250 1.643 1.510 1.577 1.445  
125 1.211 1.191 1.201 1.069  
62.5 0.832 0.784 0.808 0.676  
31.2 0.554 0.537 0.546 0.414  
15.6 0.325 0.305 0.315 0.183  
0 0.136 0.128 0.132    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat IL1RL1 ELISA Kit was designed for the quantitative measurement of Rat IL1RL1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function
(From Uniprot)
Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.; Inhibits IL-33 signaling.
Gene References into Functions
  1. administration of sST2 significantly attenuated the severity of severe acute pancreatitis and associated cardiac damage, and the cardioprotective effect is associated with its anti-inflammatory action PMID: 29653119
  2. Study demonstrates that IL-33 and ST2 are highly expressed by various central nervous system (CNS) resident cells, and there was a change of the expression levels and patterns of both molecules in the CNS lesions of multiple sclerosis (MS)patients compared with healthy brain tissues. Furthermore, IL-33/ST2 signaling pathway may have its unique important function in the myelination process in the CNS compartment. PMID: 27455844
  3. A strong relationship of IL-33/ST2 with natriuretic peptides and classical inflammatory mediators was observed in cardiac tissue. Expression of sST2 in cardiac, adipose and liver tissue decreased in obese rats compared with controls, suggesting an involvement for IL-33/ST2 system in molecular mechanisms of obesity. PMID: 28274612
  4. these results demonstrated that ST2L translocation from the cytosol to the cell membranes of lung tissue and the down-expression of sST2 in both fractions can function as new biomarkers of (ventilator-induced lung injury. PMID: 25815839
  5. This is the first study demonstrating a deleterious role for sST2 in the vascular remodeling associated with obesity. PMID: 24265755
  6. Interleukin-33 prevents apoptosis and improves survival after experimental myocardial infarction through ST2 signaling. PMID: 19919994

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.; [Isoform B]: Secreted.
Protein Families
Interleukin-1 receptor family
Tissue Specificity
Isoform A is detected in spleen, lung, bone marrow and lymh node. Isoform B is predominant in fibroblasts.
Database Links
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