Rat Indoleamine 2,3-dioxygenase,IDO ELISA Kit

Instructions
Code CSB-E13583r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name indoleamine 2,3-dioxygenase 1
Alternative Names Ido1 ELISA Kit; Ido ELISA Kit; Indo ELISA Kit; Indoleamine 2,3-dioxygenase 1 ELISA Kit; IDO-1 ELISA Kit; EC 1.13.11.52 ELISA Kit; Indoleamine-pyrrole 2,3-dioxygenase ELISA Kit
Abbreviation IDO1
Uniprot No. Q9ERD9
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IDO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %86
Range %81-92
1:2Average %98
Range %93-102
1:4Average %91
Range %85-97
1:8Average %95
Range %91-99
Recovery
The recovery of rat IDO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-93
EDTA plasma (n=4)9689-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.864 2.711 2.788 2.693
10001.852 1.724 1.788 1.693
5001.076 1.031 1.054 0.959
2500.652 0.657 0.655 0.560
1250.361 0.377 0.369 0.274
62.50.264 0.254 0.259 0.164
31.250.172 0.178 0.175 0.080
00.096 0.094 0.095
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection.
Gene References into Functions
  1. Results show the antidepressant effect of infliximab and suggest that its mechanism is partly related to inhibition of indoleamine 2, 3-dioxygenase/3-hydroxyl amino acid oxygenase pathway activation mediated by tumor necrosis factor-alpha in rat brain. PMID: 27366867
  2. this study suggests a link between melatonin, JNK, FoxO1 and IDO1 that acts as a potential balance regulator of tryptophan metabolism, and offers a new approach to treat diseases related to dysregulation of tryptophan metabolism PMID: 28118532
  3. results suggest that M30 is neuroprotective against CORT-induced depression targeting elevated MAO activities that cause oxidative stress and neuroinflammation, resulting in IDO-1 activation, serotonin deficiency and neurodegeneration. PMID: 27870896
  4. IDO expression in NSCs synergistically potentiates the immune suppression activities of NSCs and could be applicable for the development of therapeutic modalities against various neurodegenerative diseases. PMID: 26636969
  5. study demonstrates that IDO gene transfer into engineered lung allograft tissues significantly attenuates acute allograft damage suggesting local therapy with IDO as a strategy to reduce need for systemic immunosuppression and, thereby, its side effects PMID: 26506443
  6. Indoleamine 2,3-dioxygenase is upregulated in the brain of rats with acute pancreatitis. PMID: 25829217
  7. inhibitory effect of locally delivered CTLA4Ig is dependent on IDO activities within the allograft. PMID: 25498102
  8. Transfer of tolerance to naive recipients is dependent upon IDO-mediated immunosuppression. Initiators of IDO activity, CTLA-4 or soluble CTLA-4 did not mediate this tolerogenic process. PMID: 23607691
  9. results suggest IDO gene expression correlates with the severity of acute rejection and IFN-gamma induced IDO-positive DCs may attenuate acute rejection and catalyze local tryptophan metabolism via IDO enzyme expression,leading to tolerance after liver transplantation PMID: 22454246
  10. Ido1 gene knockout or pharmacological inhibition of hippocampal IDO1 activity attenuated both nociceptive and depressive behavior PMID: 22751107
  11. zebularine plus interferon gamma induce IDO1 gene expression related to demethylation events in the IDO1 promoter region and to up-regulation of expression of transcription factors PMID: 22424783
  12. Detection of IDO mRNA in peripheral blood diagnosed acute rejection in the rat model at an earlier stage. PMID: 22172881
  13. Data suggest that during the first phase of infection the IDO-mediated tryptophan depletion has a predominantly antimicrobial effect. PMID: 19602041
  14. NK cells expression is associated with liver allograft acceptance PMID: 19944758
  15. HO-1/IDO cross-regulation modulates apoptosis and proliferation in rat and human breast cancer cells PMID: 16319139
  16. In conclusion, this study demonstrates an age-related decline in Cu/Zn-SOD and IDO activities, the two enzymes responsible for scavenging O2*-. PMID: 16688932
  17. Report immunological role of indoleamine 2,3-dioxygenase in rat liver allograft rejection and tolerance. PMID: 17645734
  18. CD4+T cells accumulate at the heart transplant site and induce graft endothelial cell expression of Indoleamine 2, 3-dioxygenase (IDO) PMID: 17868070
  19. IDO inhibition for 7 days after transplantation reduces survival, but does not cause acute rejection of the liver in an immunotoleramt model. PMID: 18475196
  20. gene delivery using IDO was unsuccessful in prolonging rat liver allograft survival. PMID: 19177450
  21. In vitro, the proliferative ability of T cells or B cells from experimental autoimmune myastenia gravis rats was inhibited when they were cocultured with bone marrow stromal cells. This inhibitory effect was partially dependent on the secretion of IDO PMID: 19283707
  22. Liver-induced long-term acceptance seen in human combined auxiliary liver and kidney transplantation is at least partly mediated by InDO activity. PMID: 19935463

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Subcellular Location Cytoplasm, cytosol
Protein Families Indoleamine 2,3-dioxygenase family
Database Links

KEGG: rno:66029

STRING: 10116.ENSRNOP00000050320

UniGene: Rn.64513

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