Rat Interleukin 1 receptor antagonist, IL-1ra ELISA kit

Code CSB-E10397r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat IL1RN ELISA Kit was designed for the quantitative measurement of Rat IL1RN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

Target Name interleukin 1 receptor antagonist
Alternative Names Il1rn ELISA Kit; Il-1raInterleukin-1 receptor antagonist protein ELISA Kit; IL-1RN ELISA Kit; IL-1ra ELISA Kit; IRAP ELISA Kit; IL1 inhibitor ELISA Kit
Abbreviation IL1RN
Uniprot No. P25086
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %102
Range %98-105
1:2Average %97
Range %92-102
1:4Average %96
Range %90-101
1:8Average %93
Range %88-99
Recovery
The recovery of rat IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-104
EDTA plasma (n=4)8982-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
80002.259 2.269 2.264 2.148
40001.554 1.597 1.576 1.460
20001.040 1.068 1.054 0.938
10000.625 0.672 0.649 0.533
5000.398 0.371 0.385 0.269
2500.279 0.274 0.277 0.161
1250.181 0.189 0.185 0.069
00.114 0.118 0.116  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
Gene References into Functions
  1. IL-1beta is the key trigger of neuroinflammation and that IL-1ra prevents the neuroinflammation entering the chronic state PMID: 24621600
  2. Interleukin-1beta and interleukin-1 receptor antagonist appear in grey matter additionally to white matter during experimental multiple sclerosis PMID: 24376764
  3. Gene expression of the natural IL-1 receptor antagonist IL-1ra and IL-18 (but not TNFalpha) is increased in the ipsilateral hippocampus during long-term potentiation. PMID: 23747799
  4. the IL-1ra gene could be transferred and expressed in corneal tissue efficiently. This may be a novel technique for gene transfection to the cornea in situ. PMID: 22827713
  5. Energy-sensing factors coactivator peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha) and AMP-activated protein kinase control expression of inflammatory mediators in liver: induction of interleukin 1 receptor antagonist. PMID: 22117073
  6. IL1Ra has a role in reducing islet endothelial activation and oxidative stress gene expression in the GK diabetic rat PMID: 19742300
  7. IL-1ra, even when applied for only 4 h, reduces neuronal cell death and the number of microglial cells after excitotoxic damage PMID: 15932594
  8. rhIL-1ra may protect renal injury after asphyxia via inhibiting intrarenal inflammatory response. PMID: 16259926
  9. IL-1ra is a modulator of gender differences in the febrile response to lipopolysaccharides. PMID: 17138728
  10. physical training has an effect on IL-1beta, IL-6 and IL-1ra concentrations in various brain areas of the rat PMID: 18299269
  11. Estrogen-induced protection is associated with a decrease in IL-1beta and an increase in IL-1ra expression in the ischemic hippocampus which suggests that modulation of IL-1beta/IL-1ra might be a part of anti-inflammatory effects of estrogen. PMID: 18455872
  12. A pro-inflammatory orientation in the interleukin-1 beta/interleukin-1 receptor antagonist balance might play a role in the initiation of perinatal brain damages. PMID: 18511291

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Subcellular Location Secreted.
Protein Families IL-1 family
Database Links

KEGG: rno:60582

STRING: 10116.ENSRNOP00000007949

UniGene: Rn.162640

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