Rat Interleukin 1 receptor antagonist, IL-1ra ELISA kit

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Description

This Rat IL1RN ELISA Kit was designed for the quantitative measurement of Rat IL1RN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

Target Name

interleukin 1 receptor antagonist

Alternative Names

Il1rn ELISA Kit; Il-1raInterleukin-1 receptor antagonist protein ELISA Kit; IL-1RN ELISA Kit; IL-1ra ELISA Kit; IRAP ELISA Kit; IL1 inhibitor ELISA Kit

Abbreviation

IL1RN

Uniprot No.

P25086

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-105
1:2	Average %	97
	Range %	92-102
1:4	Average %	96
	Range %	90-101
1:8	Average %	93
	Range %	88-99

Recovery

The recovery of rat IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-104
EDTA plasma (n=4)	89	82-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.259	2.269	2.264	2.148
4000	1.554	1.597	1.576	1.460
2000	1.040	1.068	1.054	0.938
1000	0.625	0.672	0.649	0.533
500	0.398	0.371	0.385	0.269
250	0.279	0.274	0.277	0.161
125	0.181	0.189	0.185	0.069
0	0.114	0.118	0.116

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
Gene References into Functions	IL-1beta is the key trigger of neuroinflammation and that IL-1ra prevents the neuroinflammation entering the chronic state PMID: 24621600 Interleukin-1beta and interleukin-1 receptor antagonist appear in grey matter additionally to white matter during experimental multiple sclerosis PMID: 24376764 Gene expression of the natural IL-1 receptor antagonist IL-1ra and IL-18 (but not TNFalpha) is increased in the ipsilateral hippocampus during long-term potentiation. PMID: 23747799 the IL-1ra gene could be transferred and expressed in corneal tissue efficiently. This may be a novel technique for gene transfection to the cornea in situ. PMID: 22827713 Energy-sensing factors coactivator peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha) and AMP-activated protein kinase control expression of inflammatory mediators in liver: induction of interleukin 1 receptor antagonist. PMID: 22117073 IL1Ra has a role in reducing islet endothelial activation and oxidative stress gene expression in the GK diabetic rat PMID: 19742300 IL-1ra, even when applied for only 4 h, reduces neuronal cell death and the number of microglial cells after excitotoxic damage PMID: 15932594 rhIL-1ra may protect renal injury after asphyxia via inhibiting intrarenal inflammatory response. PMID: 16259926 IL-1ra is a modulator of gender differences in the febrile response to lipopolysaccharides. PMID: 17138728 physical training has an effect on IL-1beta, IL-6 and IL-1ra concentrations in various brain areas of the rat PMID: 18299269 Estrogen-induced protection is associated with a decrease in IL-1beta and an increase in IL-1ra expression in the ischemic hippocampus which suggests that modulation of IL-1beta/IL-1ra might be a part of anti-inflammatory effects of estrogen. PMID: 18455872 A pro-inflammatory orientation in the interleukin-1 beta/interleukin-1 receptor antagonist balance might play a role in the initiation of perinatal brain damages. PMID: 18511291 Show More Hide All
Subcellular Location	Secreted.
Protein Families	IL-1 family
Database Links	KEGG: rno:60582 STRING: 10116.ENSRNOP00000007949 UniGene: Rn.162640

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Check Code：

Code	CSB-E10397r
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.