Rat Interleukin 10,IL-10 ELISA KIT

Code CSB-E04595r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P29456
Lead Time 3-5 working days
Abbreviation IL10
Target Name interleukin 10
Alias CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
HGNC 5962
RGD 2886
MGI 96537
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %88-98
1:2Average %96
Range %91-101
1:4Average %90
Range %85-95
1:8Average %93
Range %90-96
Recovery
The recovery of rat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-98
EDTA plasma (n=4)9590-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.611 2.671 2.641 2.474
1001.873 1.867 1.870 1.703
501.206 1.234 1.220 1.053
250.804 0.817 0.811 0.644
12.50.515 0.527 0.521 0.354
6.250.387 0.399 0.393 0.226
3.120.314 0.327 0.321 0.154
00.155 0.179 0.167  
References
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Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location Secreted
Protein Families IL-10 family
Database Links

KEGG: rno:25325

STRING: 10116.ENSRNOP00000006246

UniGene: Rn.9868

Pathway Jak-STAT signaling pathway
T cell receptor signaling pathway
FoxO signaling pathway

Q&A and Customer Reviews

Customer Reviews
  • Sample type: Culture supernatants
    Sample species: Rat
    Review: The levels of cytokines in the different groups (n = 9). The production of IL-10 in supernatant of cultured PMNs was detected by ELISA. PMNs were cultured with NPS-2143 (100 nM), cinacalcet (100 nM), and PDTC (100 nM) for 12 h after stimulated by fMLP (10 nM) for 2 min. #P < 0 05 versus control group, ∗P < 0 05 versus fMLP group, and △P < 0 01 versus fMLP + CIN group.
    PMID: 29081886
  • Sample type: Culture supernatants
    Sample species: Rat
    Review: HO‐1 overexpression increases the production of paracrine factors from MSCs under PC stimulation. PCs were applied to all types of stem cells for 7 days, and then the levels of IL-10 (g) in culture supernatants were analyzed by enzyme-linked immunosorbent assays. All data are expressed as mean ± SEM (n = 6). *p < 0.05 versus MSC + PBS and MSC‐HO‐1 + PBS groups, #p < 0.05 versus MSC + PC group, and Δp < 0.05 versus MSC-HO-1 + PC group.
    PMID: 30362554
  • Sample type: Colorectal tissues
    Sample species: Rat
    Review: The anti-inflammatory cytokines IL-10 is decreased to a low level after TNBS exposure, whereas treatment with middle- or high-dose of BD-SNEDDS, BD-suspension, or AZA notably restored these anti-inflammatory cytokines. Data are expressed as mean±SD and analyzed by ANOVA followed by Dunnett’s test. ##P,0.01 vs intact group, **P,0.01 and *P,0.05 vs the vehicle group, P,0.05 and P,0.01 vs BD-suspension group.
    PMID: 30319255
  • Sample type: Tissues
    Sample species: Rat
    Review: IL-10 levels were obtained in treated and untreated groups (a) and comparison to the highest level obtained for each cytokine in periodontitis group (positive control).
    PMID: 30194012
  • Sample type: Culture supernatants
    Sample species: Rat
    Review: Compared with the vector-NC group, decreased  IL-10 level were found in the P2X7-vector group (p < 0.05).
    PMID: 30892077
  • Sample type: Plamsa
    Sample species: Rat
    Review: Effect of KJL on IL-1 in plasma. Data are presented as the mean ± SD. ##P < 0.01, #P < 0.05 versus the control group; **P < 0.01, *P < 0.05 versus the TNBS group. (n = 8–12).
    PMID: 28750820
  • Sample type: Liver homogenate tissue
    Sample species: Rat
    Review: Effect of PTX on BDL-induced changes in liver IL-10, fourteen days post-operation, paclitaxel (PTX) (0.3 mg/kg/ip) was administered three times a week for 2 weeks. Hepatic IL-10 level were measured thereafter. Data are expressed as mean ± SD (n=6).
    PMID: 30243650
  • Sample type: Culture supernatants
    Sample species: Rat
    Review: Nrf2 knockdown reversed the anti-inflammatory effects of SalC. Nrf2 siRNA reversed SalC-induced IL‑10 downregulation. All data are presented as mean ± SD of four independent experiments. ##P < 0.01 vs control group, ⁎⁎P < 0.01 vs LPS group, scale bar = 20 μm.
    PMID: 30142530
  • Sample type: Jejunal mucosa tissues
    Sample species: Rat
    Review: Effect of ethanolamine on cytokines of weaned rats. The concentrations of cytokines in the jejunal mucosa were not different among groups.
    PMID: 29789027
  • Sample type: Brain tissues
    Sample species: Rat
    Review: Concentrations of the IL10 and their receptors were significantly lower in the serum than in the central nervous system in both SH and WKY rats (p < 0.001).
    PMID: 30539780

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