Rat Interleukin 10,IL-10 ELISA KIT

Rat Interleukin 10,IL-10 ELISA KIT

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Il10 Proteins

Il10 Proteins

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Rat Interleukin 10,IL-10 ELISA KIT

Specification
Target

Code

CSB-E04595r

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P29456

Lead Time

3-5 working days

Abbreviation

IL10

Target Name

interleukin 10

Alias

CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

HGNC

5962

RGD

2886

MGI

96537

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-98
1:2	Average %	96
	Range %	91-101
1:4	Average %	90
	Range %	85-95
1:8	Average %	93
	Range %	90-96

Recovery

The recovery of rat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	95	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.611	2.671	2.641	2.474
100	1.873	1.867	1.870	1.703
50	1.206	1.234	1.220	1.053
25	0.804	0.817	0.811	0.644
12.5	0.515	0.527	0.521	0.354
6.25	0.387	0.399	0.393	0.226
3.12	0.314	0.327	0.321	0.154
0	0.155	0.179	0.167

References

Glutamine modifies immune responses of mice infected with porcine circovirus type 2 Ren W et al,Br J Nutr,2013 Jan 28:1-8.
Immunostimulative effects of Cyperus rotundus, Alpinia calcarata, Solanum surattense, Clerodendrum infortunatum and Croton laccifer extracts combination on cyclophosphamide-induced immunosupression in rats Ediriweera P. S. et al,Journal of Experimental and Integrative Medicine,2015 5(2): 110-113
Identification and immunogenicity of microneme protein 2 (EbMIC2) of Eimeria brunetti Hoan TD. et al,Exp Parasitol,2016 162: 7-17
Effect of Ampelopsis Radix on wound healing in scalded rats Lee K. et al,BMC Complement Altern Med,2015 Jul 8;15:213.
Magnesium isoglycyrrhizinate inhibits inflammatory response through STAT3 pathway to protect remnant liver function Tang GH. et al,World J Gastroenterol,2015 21(43):12370-80.
Protective effects of fenofibrate and resveratrol in an aggressive model of rheumatoid arthritis in rats Wahba MG. et al,Pharm Biol,2015 Dec 24:1-11.
Prednisone treatment inhibits the differentiation of B lymphocytes into plasma cells in MRL/MpSlac-lpr mice. Yan SX.et al,Acta Pharmacol Sin.,2016 36(11):1367-76.
BAFF and its receptors involved in the inflammation progress in adjuvant induced arthritis rats Wei F. et al,Int Immunopharmacol,/
Effects of Castanospermine on Inflammatory Response in a Rat Model of Experimental Severe Acute Pancreatitis Yu-pu Hong.et al,Archives of Medical Research,August
Effects of pre and post-treatments with dipyridamole in gentamicin-induced acute nephrotoxicity in the rat Pitchai Balakumar.et al,Regulatory Toxicology and Pharmacology,March
Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils Tai-yu Zhai.et al,Oxidative Medicine and Cellular Longevity,Sep 10
Ulinastatin ameliorates tissue damage of severe acute pancreatitis through modulating regulatory T cells Yu Pan.et al,Journal of Inflammation,Mar 20
Proteomic changes of CD4+/CD25+/forkhead box p3+ regulatory T cells in a 30 Yuxia Jiao.et al,Experimental and Therapeutic Medicine,Sep 29
Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq Maimaitiaili, Abudunaibi.et al,Am J Transl Res,/
Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats Jian Zhou.et al,British Journal of Nutrition,May 23
Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats YuLong.et al,Biomedicine & Pharmacotherapy,June 15
The Cu-containing TiO2 coatings with modulatory effects on macrophage polarization and bactericidal capacity prepared by micro-arc oxidation on titanium substrates QianliHuang.et al,Colloids and Surfaces B: Biointerfaces,June 19
Tumour necrosis factor and interleukin 10 in blood pressure regulation in spontaneously hypertensive and normotensive rats AgnieszkaSegiet.et al,Cytokine,July 6
Volatile Oil of Amomum villosum Inhibits Nonalcoholic Fatty Liver Disease via the Gut-Liver Axis Shanhong Lu.et al,BioMed Research International,July 19
Activation of Nrf2 signaling by salvianolic acid C attenuates NF‑κB mediated inflammatory response both in vivo and in vitro Junke Song, .et al,International Immunopharmacology,August 22
Paclitaxel alleviates liver fibrosis induced by bile duct ligation in rats: Role of TGF-β1, IL-10 and c-Myc Maha H.Sharawy.et al,Life Sciences,September 20
Prophylactic treatment with MSC derived exosomes attenuates traumatic acute lung injury in rats Li QC, et al,American Journal of Physiology-Lung Cellular and Molecular Physiology,March 20
Beneficial therapeutic effects of a Chinese herbal formula on autoimmune thyroiditis: preclinical results and outcome of a pilot clinical study Hang Ren, et al,International Journal of Clinical And Experimental Medicine,December 30
Ischemia Injury: A New Method Accelerates Bone Healing in a Rat Tibia Fracture Model Yang Liu, et al,BioMed Research International,April 16
Neuroprotective Effects of Zafirlukast, Piracetam and Their Combination on L-Methionine-Induced Vascular Dementia in Rats Fayez AM, et al,fundamental & clinical pharmacology,April 18

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Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location	Secreted
Protein Families	IL-10 family
Database Links	KEGG: rno:25325 STRING: 10116.ENSRNOP00000006246 UniGene: Rn.9868
Pathway	Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway

Il10 Protein

Il10 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP011580RA CSB-EP011580RA CSB-BP011580RA CSB-MP011580RA	Recombinant Rat Interleukin-10(Il10)	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.