Rat Interleukin 11,IL-11 ELISA KIT

Instructions
Code CSB-E07358r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 11
Alternative Names Il11 ELISA Kit; Interleukin-11 ELISA Kit; IL-11 ELISA Kit
Abbreviation IL11
Uniprot No. Q99MF5
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %81-94
1:2Average %85
Range %80-90
1:4Average %92
Range %86-101
1:8Average %95
Range %89-102
Recovery
The recovery of rat IL-11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9080-105
EDTA plasma (n=4)9890-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.179 2.116 2.148 2.055
5001.773 1.704 1.739 1.646
2501.426 1.313 1.370 1.277
1251.009 0.959 0.984 0.891
62.50.537 0.517 0.527 0.434
31.20.262 0.250 0.256 0.163
15.60.198 0.186 0.192 0.099
00.095 0.090 0.093  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3.
Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

STRING: 10116.ENSRNOP00000023489

UniGene: Rn.198483

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