Code | CSB-E07362r |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 102 | ||||
Range % | 96-106 | |||||
1:2 | Average % | 94 | ||||
Range % | 90-98 | |||||
1:4 | Average % | 95 | ||||
Range % | 90-99 | |||||
1:8 | Average % | 89 | ||||
Range % | 82-94 |
The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 98 | 92-104 | ||||
EDTA plasma (n=4) | 103 | 97-107 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The rat IL-12 ELISA kit is suitable for the quantitative determination of rat IL-12 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.
IL-12 is a Th1 cytokine predominantly produced by cells in the innate immune system. Its role is linked to the differentiation of naïve T cells into Th1 cells. In allergy, IL-12 is known to downregulate allergic inflammation following its release, along with IFNγ. It modulates the expression of many genes involved in effector T cell function such as FasL and granzyme B. IL-12 signals through the IL-12R activate a STAT4-dependent intracellular pathway, leading to the production of IFNγ but also directly resulting in IL-18 synthesis. It is important for cancer therapy to provide a crucial bridge between innate and adaptive immunity.
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