| Code |
CSB-E07362r |
| See More Details |
 |
| Product Type |
ELISA Kit |
| Size |
96T,5×96T,10×96T |
| Lead Time |
3-5 working days |
| Abbreviation |
IL-12/P40 |
| Target Name |
Interleukin 12,IL-12/P40 |
| Alias |
N/A |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Protocol |
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 102 | | | Range % | 96-106 | | | 1:2 | Average % | 94 | | | Range % | 90-98 | | | 1:4 | Average % | 95 | | | Range % | 90-99 | | | 1:8 | Average % | 89 | | | Range % | 82-94 | |
|
| Recovery |
| The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 98 | 92-104 | | | EDTA plasma (n=4) | 103 | 97-107 | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.329 | 2.228 | 2.279 | 2.077 | | | 1000 | 1.748 | 1.647 | 1.698 | 1.496 | | | 500 | 1.152 | 1.131 | 1.142 | 0.940 | | | 250 | 0.794 | 0.783 | 0.789 | 0.587 | | | 125 | 0.506 | 0.495 | 0.501 | 0.299 | | | 62.5 | 0.415 | 0.404 | 0.410 | 0.208 | | | 31.25 | 0.308 | 0.303 | 0.306 | 0.104 | | | 0 | 0.202 | 0.201 | 0.202 | | |
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| References |
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Immunostimulative effects of Cyperus rotundus, Alpinia calcarata, Solanum surattense, Clerodendrum infortunatum and Croton laccifer extracts combination on cyclophosphamide-induced immunosupression in rats Ediriweera P. S. et al,Journal of Experimental and Integrative Medicine,2015 5(2): 110-113
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High-dose dexamethasone induced LPS-stimulated rat alveolar macrophages apoptosis Zeng S.et al,Drug Des Devel Ther,Oct 25
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The immunological and protective role of Baicalin in male rats treated with chemotherapy (Gemcitabine) Maytham T. Qasim, et al,Nanoscience and Nanotechnology Letters,/
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