Rat Interleukin 12,IL-12/P40 ELISA KIT

Code CSB-E07362r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IL-12/P40
Target Name Interleukin 12,IL-12/P40
Alias N/A
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %102
Range %96-106
1:2Average %94
Range %90-98
1:4Average %95
Range %90-99
1:8Average %89
Range %82-94
Recovery
The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-104
EDTA plasma (n=4)10397-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.329 2.228 2.279 2.077
10001.748 1.647 1.698 1.496
5001.152 1.131 1.142 0.940
2500.794 0.783 0.789 0.587
1250.506 0.495 0.501 0.299
62.50.415 0.404 0.410 0.208
31.250.308 0.303 0.306 0.104
00.202 0.201 0.202  
References
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