Rat Interleukin 12,IL-12/P40 ELISA KIT

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Name: Rat Interleukin 12,IL-12/P40 ELISA KIT
Brand: CUSABIO
SKU: CSB-E07362r

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Code	CSB-E07362r
Size	96T,5×96T,10×96T
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Target Name

Interleukin 12,IL-12/P40

Alternative Names

N/A

Abbreviation

IL12/P40

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	96-106
1:2	Average %	94
	Range %	90-98
1:4	Average %	95
	Range %	90-99
1:8	Average %	89
	Range %	82-94

Recovery

The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	103	97-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.329	2.228	2.279	2.077
1000	1.748	1.647	1.698	1.496
500	1.152	1.131	1.142	0.940
250	0.794	0.783	0.789	0.587
125	0.506	0.495	0.501	0.299
62.5	0.415	0.404	0.410	0.208
31.25	0.308	0.303	0.306	0.104
0	0.202	0.201	0.202

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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The immunological and protective role of Baicalin in male rats treated with chemotherapy (Gemcitabine) Maytham T. Qasim, et al,Nanoscience and Nanotechnology Letters,2019
High-dose dexamethasone induced LPS-stimulated rat alveolar macrophages apoptosis Zeng S.et al,Drug Des Devel Ther,2017
Immunostimulative effects of Cyperus rotundus, Alpinia calcarata, Solanum surattense, Clerodendrum infortunatum and Croton laccifer extracts combination on cyclophosphamide-induced immunosupression in rats Ediriweera P. S. et al,Journal of Experimental and Integrative Medicine,2015

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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