Rat Interleukin 12,IL-12/P40 ELISA KIT

Code CSB-E07362r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Interleukin 12,IL-12/P40
Alternative Names
N/A
Abbreviation
IL12/P40
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %102
Range %96-106
1:2Average %94
Range %90-98
1:4Average %95
Range %90-99
1:8Average %89
Range %82-94
Recovery
The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-104
EDTA plasma (n=4)10397-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.329 2.228 2.279 2.077
10001.748 1.647 1.698 1.496
5001.152 1.131 1.142 0.940
2500.794 0.783 0.789 0.587
1250.506 0.495 0.501 0.299
62.50.415 0.404 0.410 0.208
31.250.308 0.303 0.306 0.104
00.202 0.201 0.202  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The rat IL-12 ELISA kit is suitable for the quantitative determination of rat IL-12 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

IL-12 is a Th1 cytokine predominantly produced by cells in the innate immune system. Its role is linked to the differentiation of naïve T cells into Th1 cells. In allergy, IL-12 is known to downregulate allergic inflammation following its release, along with IFNγ. It modulates the expression of many genes involved in effector T cell function such as FasL and granzyme B. IL-12 signals through the IL-12R activate a STAT4-dependent intracellular pathway, leading to the production of IFNγ but also directly resulting in IL-18 synthesis. It is important for cancer therapy to provide a crucial bridge between innate and adaptive immunity.

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