Rat Interleukin 12,IL-12/P40 ELISA KIT

Code CSB-E07362r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

The rat NF-κB subunit p65 ELISA kit is suitable for the quantitative determination of rat p65 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

IL-12 is a Th1 cytokine predominantly produced by cells in the innate immune system. Its role is linked to the differentiation of naïve T cells into Th1 cells. In allergy, IL-12 is known to downregulate allergic inflammation following its release, along with IFNγ. It modulates the expression of many genes involved in effector T cell function such as FasL and granzyme B. IL-12 signals through the IL-12R activate a STAT4-dependent intracellular pathway, leading to the production of IFNγ but also directly resulting in IL-18 synthesis. It is important for cancer therapy to provide a crucial bridge between innate and adaptive immunity.

Target Name Interleukin 12,IL-12/P40
Alternative Names N/A
Abbreviation IL12/P40
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %102
Range %96-106
1:2Average %94
Range %90-98
1:4Average %95
Range %90-99
1:8Average %89
Range %82-94
Recovery
The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-104
EDTA plasma (n=4)10397-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.329 2.228 2.279 2.077
10001.748 1.647 1.698 1.496
5001.152 1.131 1.142 0.940
2500.794 0.783 0.789 0.587
1250.506 0.495 0.501 0.299
62.50.415 0.404 0.410 0.208
31.250.308 0.303 0.306 0.104
00.202 0.201 0.202  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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