Rat Interleukin-34(IL34) ELISA kit

Code CSB-EL011657RA
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
interleukin 34
Alternative Names
Il34Interleukin-34 ELISA Kit; IL-34 ELISA Kit
Abbreviation
IL34
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 84-93
1:2 Average % 107
Range % 103-110
1:4 Average % 85
Range % 81-89
1:8 Average % 89
Range % 84-93
Recovery
The recovery of rat IL34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 107 103-110
EDTA plasma (n=4) 86 83-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.100 2.168 2.134 1.995
800 1.654 1.604 1.629 1.490
400 1.181 1.204 1.193 1.054
200 0.765 0.778 0.772 0.633
100 0.517 0.502 0.510 0.371
50 0.346 0.351 0.349 0.210
25 0.228 0.231 0.230 0.091
0 0.140 0.138 0.139  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat IL34 ELISA Kit was designed for the quantitative measurement of Rat IL34 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)
Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
Gene References into Functions
  1. In a rat cardiac allograft model, IL-34 induced transplant tolerance associated with a total inhibition of alloantibody production. Treatment of rats with IL-34 promoted allograft tolerance mediated by induction of CD8+ and CD4+ Tregs. PMID: 26389674
Subcellular Location
Secreted.
Protein Families
IL-34 family
Database Links
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